Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1375333..1375958 Replicon chromosome
Accession NZ_CP082120
Organism Escherichia coli strain PC-NDM34

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag K5P75_RS06705 Protein ID WP_000911329.1
Coordinates 1375560..1375958 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag K5P75_RS06700 Protein ID WP_000450524.1
Coordinates 1375333..1375560 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5P75_RS06675 (1371134) 1371134..1371604 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
K5P75_RS06680 (1371604) 1371604..1372176 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
K5P75_RS06685 (1372322) 1372322..1373200 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
K5P75_RS06690 (1373217) 1373217..1374251 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
K5P75_RS06695 (1374464) 1374464..1375177 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
K5P75_RS06700 (1375333) 1375333..1375560 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
K5P75_RS06705 (1375560) 1375560..1375958 + 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
K5P75_RS06710 (1376105) 1376105..1376968 + 864 WP_001267507.1 neutral zinc metallopeptidase -
K5P75_RS06715 (1376983) 1376983..1378998 + 2016 WP_000829332.1 tRNA cytosine(34) acetyltransferase TmcA -
K5P75_RS06720 (1379072) 1379072..1379770 + 699 WP_000679812.1 esterase -
K5P75_RS06725 (1379880) 1379880..1380080 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T214116 WP_000911329.1 NZ_CP082120:1375560-1375958 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T214116 NZ_CP109968:c328333-328064 [Agrobacterium salinitolerans]
GTGATCTGGACAATTGAATATCACACGCTTGTCCAGAAAGAGATGCGCAAGATAAATCCCGAAGTGCGCCGGCGTATCCG
CAGCTTTCTCCATGAAAGGCCGGCGGCGCTGGACGACCCGCGCCAAACCGGCGCGGCCCTACAGGGTTCCGAACTCGGAA
ACTACTGGCGTTACCGGGTGGGCGATTACCGCATCATCTGCGATATACAGGACCACAAGCTGGTCGTTCTGGTGGTCGAA
ATCGGCCATCGCCGTGAAATTTACCGTTAG

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT214116 WP_000450524.1 NZ_CP082120:1375333-1375560 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT214116 NZ_CP109968:c328544-328317 [Agrobacterium salinitolerans]
ATGAGCAAACAGACGGCCATCCGCCTGCCTGATGAGACCTATGAGCGGCTGAAGGCGCTGTCTGAGCGCACGGGCCGCAC
ATCCGCTTACTATATCCGCGAGGCGATCGAGAAACATATCGAGGATATGGAAGACCTTTATCTTGCAGAAGAGGCGACGC
GCCGCATTCAGAACGGTGAATCGAAGGTAATAAGCGCCGAGGAGTTCTGGCGTGATCTGGACAATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References