Detailed information of TA system TA021407

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2978971..2980622
• Replicon: chromosome
• Accession: NC_007722
• Organism: Erythrobacter litoralis HTCC2594

Toxin (Protein)

• Gene name: HipA3
• Uniprot ID: Q2N5J6
• Locus tag: EL2594_RS14510
• Protein ID: WP_011415867.1
• Coordinates: 2978971..2980266 (-)
• Length: 432 a.a.

Antitoxin (Protein)

• Gene name: HipB3
• Uniprot ID: -
• Locus tag: EL2594_RS15225
• Protein ID: WP_081432332.1
• Coordinates: 2980263..2980622 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EL2594_RS14500	2975616..2977697	+	2082	WP_011415865.1	BREX system Lon protease-like protein BrxL	-
EL2594_RS14505	2977731..2978867	+	1137	WP_011415866.1	DUF4268 domain-containing protein	-
EL2594_RS14510	2978971..2980266	-	1296	WP_011415867.1	type II toxin-antitoxin system HipA family toxin	Toxin
EL2594_RS15225	2980263..2980622	-	360	WP_081432332.1	helix-turn-helix domain-containing protein	Antitoxin
EL2594_RS14520	2980775..2980975	+	201	WP_011415868.1	hypothetical protein	-
EL2594_RS14525	2980976..2983678	-	2703	WP_011415869.1	conjugal transfer protein TraG N-terminal domain-containing protein	-
EL2594_RS14530	2983691..2985127	-	1437	WP_041685409.1	conjugal transfer protein TraH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2937623..3035310	97687

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (6-115)

Antitoxin

• Cro/C1-type helix-turn-helix domain (20-76)

Sequences

Toxin

Protein

• Length: 432 a.a.
• Molecular weight: 48030.12 Da
• Isoelectric Point: 5.2204

>T21407 WP_011415867.1 NC_007722:c2980266-2978971 [Erythrobacter litoralis HTCC2594]
MSRAVVNLWGRQIGAILWDENRDVGVFEYTPEFARSGIEVAPLTMPLRGGVYDFPALNYETFKGLPGMLADSLPDKFGNA
LINRWLAEQGRTTDSFDPVERLCYTGRRGMGALEFEPSTGERREQGGPVDIAPLVDLANRVLAAREELSGVLKGDDDHHA
LQEILRVGTSAGGARAKAVLAWNEETGEFHSGQLTAGPGYTQWLVKFDGVSGNADKELADPMGFGRLEYACYLLARKAGI
DMARSRLHEEGGRAHFMTQRFDRTLDGKKLHMQSLCAMRHFDFNLARAYSYEQAIETIRMLGLGRDAIKEQVRRAFFNIF
IRNQDDHTKNIAFLMDRAGRWSLSPAYDVVYAYNPDGDWTNEHQMSLSGKTDNFELDDLISFGRFADLKIGETKAIISDI
QSAIAQWADVTSEAGVPQSMAQQALNGFRPI

Nucleotide

• Length: 1296 bp

>T21407 NC_007722:c2980266-2978971 [Erythrobacter litoralis HTCC2594]
ATGAGCCGCGCCGTTGTAAACCTGTGGGGACGGCAGATCGGCGCAATTCTGTGGGATGAGAACCGCGATGTCGGAGTATT
CGAGTATACCCCAGAGTTCGCCCGAAGCGGTATCGAAGTGGCGCCGCTCACTATGCCGCTTCGAGGCGGCGTTTATGACT
TTCCTGCACTGAACTATGAGACCTTCAAGGGTCTGCCGGGAATGCTCGCTGACAGTCTACCCGATAAGTTCGGCAACGCC
CTCATCAACCGATGGCTCGCTGAACAGGGCCGAACAACTGACAGCTTCGATCCGGTTGAACGGCTCTGCTACACCGGTCG
CAGAGGCATGGGCGCGCTTGAGTTCGAGCCATCGACAGGCGAGCGACGCGAACAGGGCGGACCCGTCGACATTGCGCCGC
TTGTTGACCTGGCCAATCGAGTACTTGCTGCAAGAGAAGAGCTCTCTGGCGTCCTCAAGGGCGATGATGATCATCATGCT
CTTCAGGAAATCCTGCGCGTCGGCACCTCCGCCGGTGGCGCGCGCGCCAAGGCCGTTCTCGCCTGGAACGAGGAAACGGG
CGAATTCCATTCTGGCCAGCTGACAGCAGGTCCCGGCTACACCCAATGGCTTGTCAAATTCGATGGCGTATCGGGCAATG
CCGACAAGGAGCTCGCCGACCCCATGGGCTTCGGGCGTCTTGAATACGCATGTTATCTGCTAGCAAGGAAGGCCGGCATC
GACATGGCCCGTTCCCGCCTGCACGAAGAAGGCGGGCGAGCCCATTTCATGACGCAGCGGTTCGACCGTACGCTCGACGG
TAAGAAGCTTCACATGCAGTCGCTTTGCGCGATGCGTCACTTCGATTTCAACCTCGCTCGTGCGTACAGCTACGAGCAAG
CGATTGAGACCATCCGCATGCTTGGGCTTGGTCGTGATGCCATCAAAGAGCAGGTGCGACGAGCGTTTTTCAACATCTTC
ATCCGCAACCAGGACGACCACACCAAGAACATCGCTTTTCTTATGGACCGAGCTGGGCGTTGGAGCCTCTCGCCTGCCTA
CGATGTTGTTTATGCCTATAATCCTGACGGGGACTGGACCAACGAGCACCAGATGTCGCTTTCAGGGAAGACAGACAATT
TCGAGCTGGACGACCTGATATCGTTTGGTCGGTTTGCAGATCTCAAAATTGGCGAAACCAAAGCAATCATTTCGGATATC
CAGTCCGCCATTGCTCAATGGGCTGATGTGACCAGTGAAGCAGGAGTGCCCCAATCGATGGCGCAGCAGGCTCTCAACGG
GTTCAGGCCCATCTAG

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13369.88 Da
• Isoelectric Point: 4.7492

>AT21407 WP_081432332.1 NC_007722:c2980622-2980263 [Erythrobacter litoralis HTCC2594]
MMHYNSSDLRPLSVLLSDFGQQLEAYRISRNLKQAELAELAGISRSTLSRLESGNGGTIDSLARIMRALDIEDRLLDIVP
DARLSPLDPRSDTGRARQRVRKSSTNEPSEDWSWDDEAP

Nucleotide

• Length: 360 bp

>AT21407 NC_007722:c2980622-2980263 [Erythrobacter litoralis HTCC2594]
ATGATGCATTACAACTCATCAGACTTGCGTCCGTTATCGGTGCTCCTGTCCGATTTTGGGCAACAGCTCGAAGCCTATCG
TATATCACGCAATCTCAAACAGGCCGAGCTTGCAGAACTAGCGGGAATTTCGCGTTCGACACTTTCCCGCTTGGAATCAG
GCAACGGCGGCACAATCGATAGCCTTGCCAGGATCATGCGGGCGCTCGATATCGAAGATCGCCTTCTCGATATCGTACCC
GACGCTAGGTTGAGCCCGCTTGACCCGCGATCGGACACGGGCAGAGCGCGGCAGCGAGTGCGGAAATCATCAACAAACGA
GCCAAGTGAAGACTGGAGTTGGGATGACGAAGCCCCATGA

Structures

Toxin

• AlphaFold DB: Q2N5J6