Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4798684..4799494 Replicon chromosome
Accession NZ_CP081820
Organism Klebsiella pneumoniae strain F726925

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag K5O13_RS23750 Protein ID WP_002887280.1
Coordinates 4798684..4799217 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag K5O13_RS23755 Protein ID WP_002887278.1
Coordinates 4799228..4799494 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5O13_RS23745 (K5O13_23745) 4797515..4798636 + 1122 WP_002887282.1 cupin domain-containing protein -
K5O13_RS23750 (K5O13_23750) 4798684..4799217 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
K5O13_RS23755 (K5O13_23755) 4799228..4799494 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
K5O13_RS23760 (K5O13_23760) 4799597..4801030 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
K5O13_RS23765 (K5O13_23765) 4801020..4801703 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
K5O13_RS23770 (K5O13_23770) 4801875..4803260 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
K5O13_RS23775 (K5O13_23775) 4803278..4803622 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T213504 WP_002887280.1 NZ_CP081820:c4799217-4798684 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T213504 NZ_CP109606:4217215-4217781 [Pseudomonas putida]
ATGCCAAACCACAACCTTCATGGAAAACTGGACTTCCTGCGCCAGGCAGAAAAGCTCAAGAACGTAACCCGCAGTGCCCA
CACCTCGACAGGCCGGCGTGAAAGCACCGCCGAGCACAGTTGGCGGCTGGCGTTGCTGGCGCTGGTGTTCGAACAAGAAC
TGGGTGATGTCGATATCTGCAAGGTGCTCAAGCTATGCCTGGTCCATGACCTTGGGGAAGCGCTGAGTGGTGATGTACCT
GCGCCGCAAGCGCATGCGGTTCCAGACAAAGGGACGAATGAGCGGCAGGACCTGGTTGCCATGACTTCAATGCTGGAACC
CTCGATGCAGGACAGCATCGTTGGCTTGTTCGATGAGTACGAAGCGGCCAGTACGCCAGAGGCCAAAGTGGTCAAGGCCT
TGGACAAAATCGAGACCCTGCTGCAACACACCCAAGGCGATAACCCGCCCGGTTTCGACTATGCGTTCAATCTTGAGTAT
GGCCGACGCTATACCGATGCCATACCGTTCCTGGCCTCGCTGAGGCGAGTAATCGATGAGGAAACACGCAAACGCCTGAA
TAGCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT213504 WP_002887278.1 NZ_CP081820:c4799494-4799228 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT213504 NZ_CP109606:4216581-4217150 [Pseudomonas putida]
ATGAATACCTCACCTTCACTTCGCAGCACCCCCATCGGCCCCCTGCTAAAGCACTGGCGCCTGTTGAACCGGGTCAAACA
GCTGCATGCCGCGCAGATGTTCGGGGTGTGCCAGTCGACCATTTCTCGCTGGGAGTCAGGCGCGCAGGACGCCGAGCCCG
AGCAACGGGCGCGTATCGAAGCCTTGCTCAAAGCCAAGCTCAGCAGCGCCGCGGATCACGCGTTGGCGCGCCTTGTGTCG
GGCAGCTCGCAACCGGTGCACTTGGTGTGCGACCTTACCCACCGCCTGCTGGCCTGCTCGCCTTCCCGCGAAGCTGAGTT
CAGTGTGCCCATCAGTGATTTGCTGGGTGTCTCGCTGTGGCCATTCGCGACAGAGGAAATCGCCAGCCAGGAACAGCGCT
TGGCAGGCATCGGCTGGCACGACAACCAGGCCTCCCCGGCGCTGGAGTTCACTACTGGCACGAATGACTCGGCACTGGTG
CCCATCCAACACAGCCTATGCCGTTGGACGCGGTTCACCCTGTCGGATGGCTCGACCGCCAGGCTGGTCGAAACCCTCGC
GCATATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References