Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4201026..4201621 Replicon chromosome
Accession NZ_CP081813
Organism Shigella flexneri strain WW1

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag K5O10_RS20160 Protein ID WP_000239579.1
Coordinates 4201026..4201376 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag K5O10_RS20165 Protein ID WP_001223208.1
Coordinates 4201370..4201621 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5O10_RS20140 (4196302) 4196302..4197324 - 1023 WP_001367906.1 ABC transporter permease -
K5O10_RS20145 (4197338) 4197338..4198840 - 1503 WP_000205794.1 sugar ABC transporter ATP-binding protein -
K5O10_RS20150 (4199150) 4199150..4200106 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
K5O10_RS20155 (4200416) 4200416..4200946 + 531 WP_000055075.1 inorganic diphosphatase -
K5O10_RS20160 (4201026) 4201026..4201376 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
K5O10_RS20165 (4201370) 4201370..4201621 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
K5O10_RS20170 (4201833) 4201833..4202174 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
K5O10_RS20175 (4202177) 4202177..4205956 - 3780 WP_000060896.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T213468 WP_000239579.1 NZ_CP081813:c4201376-4201026 [Shigella flexneri]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T213468 NZ_CP109601:2638562-2638669 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT213468 WP_001223208.1 NZ_CP081813:c4201621-4201370 [Shigella flexneri]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT213468 NZ_CP109601:c2638515-2638449 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References