Detailed information of TA system TA213354

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeV-yafW/CbtA-CbeA
• Location: 4476008..4476801
• Replicon: chromosome
• Accession: NZ_CP081767
• Organism: Escherichia coli strain 0H18

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1EZ92
• Locus tag: J6O59_RS22020
• Protein ID: WP_000854726.1
• Coordinates: 4476424..4476801 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EBQ7
• Locus tag: J6O59_RS22015
• Protein ID: WP_001548930.1
• Coordinates: 4476008..4476334 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O59_RS21970 (4471495)	4471495..4472139	-	645	WP_001547746.1	TetR/AcrR family transcriptional regulator	-
J6O59_RS21980 (4473226)	4473226..4473462	+	237	WP_001144031.1	protein YpjK	-
J6O59_RS21985 (4473462)	4473462..4473905	+	444	WP_001547764.1	lipoprotein YafY	-
J6O59_RS21990 (4473928)	4473928..4474395	+	468	WP_001547765.1	protein YkfB	-
J6O59_RS21995 (4474472)	4474472..4474711	+	240	WP_000194654.1	DUF905 family protein	-
J6O59_RS22000 (4474809)	4474809..4475267	+	459	WP_001548150.1	antirestriction protein	-
J6O59_RS22005 (4475283)	4475283..4475759	+	477	WP_000811693.1	RadC family protein	-
J6O59_RS22010 (4475768)	4475768..4475989	+	222	WP_000691994.1	DUF987 domain-containing protein	-
J6O59_RS22015 (4476008)	4476008..4476334	+	327	WP_001548930.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
J6O59_RS22020 (4476424)	4476424..4476801	+	378	WP_000854726.1	TA system toxin CbtA family protein	Toxin
J6O59_RS22025 (4476798)	4476798..4477286	+	489	WP_001547769.1	DUF5983 family protein	-
J6O59_RS22030 (4477298)	4477298..4477495	+	198	WP_000839269.1	DUF957 domain-containing protein	-
J6O59_RS22035 (4477580)	4477580..4478422	+	843	WP_001547770.1	DUF4942 domain-containing protein	-
J6O59_RS22040 (4479171)	4479171..4480709	+	1539	WP_001187177.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4440872..4488522	47650
	-	flank	IS/Tn	-	-	4472414..4472791	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14164.10 Da
• Isoelectric Point: 7.8045

>T213354 WP_000854726.1 NZ_CP081767:4476424-4476801 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T213354 NZ_CP107573:c3940181-3939882 [Pantoea dispersa]
ATGCAAACGGAACCGGAGTTTGAGTGGGATACCACCAAGGCGGAAACAAATTTCCGCAAGCATGGTATCCGCTTTGAAGT
TGCAGCCCGCGTGTTTGAGGATCCTTTCCACCTGTCGGTGCAGGATCGTTATGAAAATGGGGAATATCGCTGGCAAACCA
TTGGTAATGTTATGGGGCATGTGGTCATTCTGGTGGCCCATACGGTGCGCTTCGAAACAGATGTTGAGCGAATAAGGATT
ATCAGCGCACGCCATGCAACGCGTATTGAGAGGAGGCGCTATGAGCAAAGTCAGGTTTAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12301.97 Da
• Isoelectric Point: 7.0273

>AT213354 WP_001548930.1 NZ_CP081767:4476008-4476334 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTFGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 327 bp

>AT213354 NZ_CP107573:c3939901-3939599 [Pantoea dispersa]
ATGAGCAAAGTCAGGTTTAAACGCAGTGAATTACCGCCGCTCACCGATAAGCAACAGGCGGAGCTGGATGCTTTGAGCCG
GAAACAGGACAGCGACATCCAGTGTGATGATATTGCGCCGTTAGACGACAGTTTCTGGCAGGCTGCCGAACGGGGGCGTT
TTTACCGCCCGGTAAAAACCCAGGCTTCGATCAGAATCGATGCGGATATTCTTGAGTGGTTAAAGGCGCCTGGCAAAGGT
TACCAGACGCGCCTCAACGCGATCCTGCGAGCCGCGATGCTAAGAGAACTGCAGGGTAAATAG

Structures

Toxin

• AlphaFold DB: S1EZ92

Antitoxin

• AlphaFold DB: S1EBQ7