Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4144774..4145324 Replicon chromosome
Accession NZ_CP081762
Organism Escherichia coli strain CX21

Toxin (Protein)


Gene name ccdB Uniprot ID D7Y6T6
Locus tag J6O46_RS20375 Protein ID WP_001160971.1
Coordinates 4144774..4145088 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag J6O46_RS20380 Protein ID WP_000125566.1
Coordinates 4145091..4145324 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O46_RS20350 (4140449) 4140449..4141735 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
J6O46_RS20355 (4141735) 4141735..4142724 + 990 WP_000241253.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
J6O46_RS20360 (4142721) 4142721..4143542 + 822 WP_001065392.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
J6O46_RS20365 (4143545) 4143545..4143922 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
J6O46_RS20370 (4143929) 4143929..4144777 + 849 WP_000257189.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
J6O46_RS20375 (4144774) 4144774..4145088 - 315 WP_001160971.1 CcdB family protein Toxin
J6O46_RS20380 (4145091) 4145091..4145324 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
J6O46_RS20385 (4145410) 4145410..4145889 - 480 WP_001487721.1 type 3 dihydrofolate reductase -
J6O46_RS20390 (4146081) 4146081..4147943 - 1863 WP_223610817.1 glutathione-regulated potassium-efflux system protein KefC -
J6O46_RS20395 (4147936) 4147936..4148466 - 531 WP_000600718.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
J6O46_RS20400 (4148577) 4148577..4149908 - 1332 WP_001183198.1 MFS transporter -
J6O46_RS20405 (4149966) 4149966..4150253 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11741.75 Da        Isoelectric Point: 9.0851

>T213321 WP_001160971.1 NZ_CP081762:c4145088-4144774 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T213321 NZ_CP107524:c2113558-2113304 [Streptococcus agalactiae]
ATGTTTAATTTTACTGAAGAAGCATGGAAAGATTATGTTTCTTGGCAACAGGAAGATAAAAAAATCTTGAAACGAATTAA
TCGTTTGATAGAAGATATTAAAAGAGACCCATTTGAAGGCATTGGAAAACCAGAACCTTTAAAATACCATTATTCAGGGG
CTTGGTCTCGAAGAATTACAGAAGAACATAGGTTGATTTATATGATAGAAGATGGAGAAATCTATTTTTTATCTTTTAGA
GATCATTATAAGTAG

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT213321 WP_000125566.1 NZ_CP081762:c4145324-4145091 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT213321 NZ_CP107524:c2113817-2113551 [Streptococcus agalactiae]
ATGGAGGCTATTGTTTATTCACATTTTAGAAATAATTTAAAAGATTATATGAAGAAGGTCAATGATGAATTTGAGCCTTT
GATTGTAGTTAATAAAAATCCTGATGAAAATATAGTGGTTTTATCTCAAGATAGCTGGGAAAGTTTACAAGAAACGATTC
GTTTGATGGAAAATGATTATCTATCACATAAGGTTATTAATGGTATATCACAAGTTAAAGAGAAGCAAGTCACTAAACAT
GGTTTAATTGAGGTAGAAGATGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A241QVQ9

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References