Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 949946..950600 Replicon chromosome
Accession NZ_CP081706
Organism Escherichia coli strain AH04

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag J6O21_RS04850 Protein ID WP_000244777.1
Coordinates 950193..950600 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag J6O21_RS04845 Protein ID WP_000354046.1
Coordinates 949946..950212 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O21_RS04820 (945115) 945115..945858 + 744 WP_000951948.1 SDR family oxidoreductase -
J6O21_RS04825 (945915) 945915..947348 - 1434 WP_223566651.1 6-phospho-beta-glucosidase BglA -
J6O21_RS04830 (947393) 947393..947704 + 312 WP_001182947.1 N(4)-acetylcytidine aminohydrolase -
J6O21_RS04835 (947868) 947868..948527 + 660 WP_000250274.1 hemolysin III family protein -
J6O21_RS04840 (948723) 948723..949703 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
J6O21_RS04845 (949946) 949946..950212 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
J6O21_RS04850 (950193) 950193..950600 + 408 WP_000244777.1 protein YgfX Toxin
J6O21_RS04855 (950640) 950640..951161 - 522 WP_001055874.1 flavodoxin FldB -
J6O21_RS04860 (951273) 951273..952169 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
J6O21_RS04865 (952194) 952194..952904 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
J6O21_RS04870 (952910) 952910..954643 + 1734 WP_033816119.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T213131 WP_000244777.1 NZ_CP081706:950193-950600 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T213131 NZ_CP107468:877170-877577 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT213131 WP_000354046.1 NZ_CP081706:949946-950212 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT213131 NZ_CP107468:876923-877189 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References