Detailed information of TA system TA213096

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 715795..716582
• Replicon: chromosome
• Accession: NZ_CP081701
• Organism: Escherichia coli strain AH02

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A6L5EGB6
• Locus tag: J6O02_RS03550
• Protein ID: WP_032669201.1
• Coordinates: 716205..716582 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A6L5EE19
• Locus tag: J6O02_RS03545
• Protein ID: WP_023567998.1
• Coordinates: 715795..716154 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O02_RS03500 (711055)	711055..711768	+	714	WP_032669194.1	WYL domain-containing protein	-
J6O02_RS03505 (711765)	711765..712400	+	636	WP_020837117.1	hypothetical protein	-
J6O02_RS03510 (712439)	712439..712894	+	456	WP_000754598.1	hypothetical protein	-
J6O02_RS03515 (712891)	712891..713340	+	450	WP_032669197.1	IrmA family protein	-
J6O02_RS03520 (713412)	713412..713643	+	232	Protein_690	DUF905 domain-containing protein	-
J6O02_RS03525 (713742)	713742..714564	+	823	Protein_691	DUF945 domain-containing protein	-
J6O02_RS03530 (714595)	714595..715038	+	444	WP_032669199.1	antirestriction protein	-
J6O02_RS03535 (715050)	715050..715529	+	480	WP_032669200.1	DNA repair protein RadC	-
J6O02_RS03540 (715550)	715550..715771	+	222	WP_023567997.1	DUF987 domain-containing protein	-
J6O02_RS03545 (715795)	715795..716154	+	360	WP_023567998.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
J6O02_RS03550 (716205)	716205..716582	+	378	WP_032669201.1	TA system toxin CbtA family protein	Toxin
J6O02_RS03555 (716579)	716579..717070	+	492	WP_032672023.1	DUF5983 family protein	-
J6O02_RS03560 (717100)	717100..717303	+	204	WP_032669203.1	DUF957 domain-containing protein	-
J6O02_RS03565 (717367)	717367..718215	+	849	WP_032669204.1	DUF4942 domain-containing protein	-
J6O02_RS03575 (718518)	718518..719024	+	507	WP_000228937.1	G/U mismatch-specific DNA glycosylase	-
J6O02_RS03580 (719103)	719103..720944	-	1842	WP_000437371.1	RNA polymerase sigma factor RpoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (15-114)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14157.93 Da
• Isoelectric Point: 6.2236

>T213096 WP_032669201.1 NZ_CP081701:716205-716582 [Escherichia coli]
MQTQPLSSTQEATPRPSPVEIWQRLLSHLLDRHYGLTLNDTPFGNDGVIQEHIDAGISLCDAVNFIVEKYDLVRTDRRGF
NAETQSPLLSSIDILRARKATGLMTRNDYRTVTDITTGKYREVQP

Nucleotide

• Length: 378 bp

>T213096 NZ_CP107457:1088-1354 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13192.04 Da
• Isoelectric Point: 6.3122

>AT213096 WP_023567998.1 NZ_CP081701:715795-716154 [Escherichia coli]
MSNEIPTVNHDITEPWWGLKRSITPCFGARLVQAGNRLHYLADRANITGQFSDADLRYLDQAFPLLLKQLELMLTSGELN
PCHQHCVTLYAKGLTCEADSLGSHGYVYLAIYPTPAATA

Nucleotide

• Length: 360 bp

>AT213096 NZ_CP107457:880-1104 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Toxin

• AlphaFold DB: A0A6L5EGB6

Antitoxin

• AlphaFold DB: A0A6L5EE19