Detailed information of TA system TA212752

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2344054..2345608
• Replicon: chromosome
• Accession: NZ_CP081212
• Organism: Phaeobacter inhibens strain M624

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: K4F87_RS11035
• Protein ID: WP_260081754.1
• Coordinates: 2344054..2345355 (-)
• Length: 434 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: K4F87_RS11040
• Protein ID: WP_108800536.1
• Coordinates: 2345357..2345608 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K4F87_RS11010 (K4F87_10985)	2339283..2340479	-	1197	WP_260081750.1	AAA family ATPase	-
K4F87_RS11015 (K4F87_10990)	2340480..2341700	-	1221	WP_260081751.1	restriction endonuclease subunit S	-
K4F87_RS11020 (K4F87_10995)	2341697..2343244	-	1548	WP_260081752.1	type I restriction-modification system subunit M	-
K4F87_RS11025 (K4F87_11000)	2343241..2343435	-	195	WP_058239057.1	helix-turn-helix domain-containing protein	-
K4F87_RS11030 (K4F87_11005)	2343524..2343781	-	258	WP_260081753.1	hypothetical protein	-
K4F87_RS11035 (K4F87_11010)	2344054..2345355	-	1302	WP_260081754.1	type II toxin-antitoxin system HipA family toxin	Toxin
K4F87_RS11040 (K4F87_11015)	2345357..2345608	-	252	WP_108800536.1	helix-turn-helix transcriptional regulator	Antitoxin
K4F87_RS11045 (K4F87_11020)	2345748..2346611	-	864	WP_260081755.1	hypothetical protein	-
K4F87_RS11050 (K4F87_11030)	2348234..2349070	-	837	WP_014875225.1	DeoR/GlpR family DNA-binding transcription regulator	-
K4F87_RS11055 (K4F87_11035)	2349287..2350234	+	948	WP_014875226.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2308355..2346908	38553

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (10-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-67)

Sequences

Toxin

Protein

• Length: 434 a.a.
• Molecular weight: 48147.90 Da
• Isoelectric Point: 5.5052

>T212752 WP_260081754.1 NZ_CP081212:c2345355-2344054 [Phaeobacter inhibens]
MGRKRSYTPLNVFLNSRLVGQLVREPSGGVSFAYAHDWLEWEHRMPVSLSLPLQENRYSGAPVMAVFDNLLPDSDLIRRR
VAERVGAEGVDAFSLLSQIGRDCIGALQFLPDGQEPQPMSELTGEPVDEAQIGAILGDLDLAPLGIRRENDFRISVAGAQ
EKTALLRKDGQWIEPTGTTPTTHIIKPQIGRLPNGMDLSDSVENEYLCLKLMEAFGLRATNVEMAQFGDKKALVVERFDR
RWTSDGRLIRLPQEDCCQALSVVPTQKYQNEGGPGISDIMDLLLGSDDPNKDRYDFFKTNVLFWLLGATDGHGKNFSVSL
LPGGRFRMTPLYDVLTVQPTVDARQIERKYFKLAMNFGNSNHYKVANIVGRHIVETGVKSGLSRAVVQGLFEELQETSHA
IVEATFNQLPKGFPEELLASIDAALKARLHLLN

Nucleotide

• Length: 1302 bp

>T212752 NZ_CP107345:c5364457-5364074 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9114.40 Da
• Isoelectric Point: 8.7278

>AT212752 WP_108800536.1 NZ_CP081212:c2345608-2345357 [Phaeobacter inhibens]
MKHLVRTSKNLGHAIRQARKAKNLTQKELASMSGVWQETISKIENGSGGTKLETVFDLIAALDLEITVTERSKGTSDGLE
DIF

Nucleotide

• Length: 252 bp

>AT212752 NZ_CP107345:c5364699-5364457 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA