Detailed information of TA system TA212713

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4264467..4265281
• Replicon: chromosome
• Accession: NZ_CP081192
• Organism: Escherichia coli strain EC1722-1

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: K4F91_RS20615
• Protein ID: WP_001054376.1
• Coordinates: 4264467..4264724 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: K4F91_RS20620
• Protein ID: WP_001309181.1
• Coordinates: 4264736..4265281 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K4F91_RS20595 (4259755)	4259755..4260861	+	1107	WP_001309184.1	N-acetylneuraminate epimerase	-
K4F91_RS20600 (4260926)	4260926..4261906	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
K4F91_RS26800 (4262016)	4262016..4262221	+	206	Protein_4032	HNH endonuclease	-
K4F91_RS20605 (4262489)	4262489..4263729	-	1241	Protein_4033	helicase YjhR	-
K4F91_RS26805 (4263845)	4263845..4263976	+	132	WP_001309182.1	hypothetical protein	-
K4F91_RS20615 (4264467)	4264467..4264724	+	258	WP_001054376.1	YjhX family toxin	Toxin
K4F91_RS20620 (4264736)	4264736..4265281	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
K4F91_RS20625 (4265337)	4265337..4266083	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
K4F91_RS20630 (4266252)	4266252..4266470	+	219	Protein_4038	hypothetical protein	-
K4F91_RS20635 (4266508)	4266508..4266624	+	117	Protein_4039	VOC family protein	-
K4F91_RS20640 (4266869)	4266869..4267990	+	1122	WP_000010829.1	M42 family metallopeptidase	-
K4F91_RS20645 (4267987)	4267987..4268265	+	279	WP_000722973.1	PTS sugar transporter subunit IIB SgcB	-
K4F91_RS20650 (4268277)	4268277..4269590	+	1314	WP_000460843.1	PTS sugar transporter subunit IIC SgcC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimI / fimA / fimE / fimB	4254252..4280971	26719

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T212713 WP_001054376.1 NZ_CP081192:4264467-4264724 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T212713 NZ_CP107334:882446-882853 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT212713 WP_001309181.1 NZ_CP081192:4264736-4265281 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT212713 NZ_CP107334:882199-882465 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8