Detailed information of TA system TA021271

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 167772..168296
• Replicon: plasmid pREL1
• Accession: NC_007491
• Organism: Rhodococcus erythropolis PR4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q3L9J5
• Locus tag: RER_RS00870
• Protein ID: WP_011331619.1
• Coordinates: 167994..168296 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: Q3L9J6
• Locus tag: RER_RS00865
• Protein ID: WP_011331620.1
• Coordinates: 167772..167993 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RER_RS00840	163267..164505	+	1239	WP_011331626.1	hypothetical protein	-
RER_RS00845	164466..164696	+	231	WP_050768542.1	hypothetical protein	-
RER_RS00850	164693..165880	+	1188	WP_041814195.1	hypothetical protein	-
RER_RS00855	165950..167251	+	1302	WP_041814197.1	AAA family ATPase	-
RER_RS33730	167281..167631	+	351	Protein_173	hypothetical protein	-
RER_RS00865	167772..167993	+	222	WP_011331620.1	hypothetical protein	Antitoxin
RER_RS00870	167994..168296	+	303	WP_011331619.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
RER_RS00875	168325..170280	+	1956	WP_041814200.1	hypothetical protein	-
RER_RS00880	170280..171077	+	798	WP_011331616.1	hypothetical protein	-
RER_RS00885	171205..172275	-	1071	WP_011331615.1	DUF1016 family protein	-
RER_RS32550	172995..173240	+	246	WP_011331613.1	integrase core domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..271577	271577

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 12963.60 Da
• Isoelectric Point: 12.8000

>T21271 WP_011331619.1 NC_007491:167994-168296 [Rhodococcus erythropolis PR4]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 303 bp

>T21271 NC_007491:167994-168296 [Rhodococcus erythropolis PR4]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 9502.90 Da
• Isoelectric Point: 12.6633

>AT21271 WP_011331620.1 NC_007491:167772-167993 [Rhodococcus erythropolis PR4]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 222 bp

>AT21271 NC_007491:167772-167993 [Rhodococcus erythropolis PR4]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Structures

Toxin

• AlphaFold DB: Q3L9J5

Antitoxin

• AlphaFold DB: Q3L9J6