Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 240942..241584 Replicon chromosome
Accession NZ_CP081175
Organism Bacillus anthracis strain P04210076

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag K0029_RS01325 Protein ID WP_000635963.1
Coordinates 241234..241584 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag K0029_RS01320 Protein ID WP_000004570.1
Coordinates 240942..241229 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K0029_RS01295 (K0029_01295) 236258..237220 + 963 WP_000961037.1 UV DNA damage repair endonuclease UvsE -
K0029_RS01300 (K0029_01300) 237213..237785 - 573 WP_000906916.1 rhomboid family intramembrane serine protease -
K0029_RS01305 (K0029_01305) 237878..238237 + 360 WP_000635040.1 holo-ACP synthase -
K0029_RS01310 (K0029_01310) 238394..239344 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
K0029_RS01315 (K0029_01315) 239463..240632 + 1170 WP_000390596.1 alanine racemase -
K0029_RS01320 (K0029_01320) 240942..241229 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
K0029_RS01325 (K0029_01325) 241234..241584 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
K0029_RS01330 (K0029_01330) 241652..243820 + 2169 WP_000426225.1 Tex family protein -
K0029_RS01335 (K0029_01335) 243878..243994 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
K0029_RS01340 (K0029_01340) 244190..244648 + 459 WP_000344248.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T212638 WP_000635963.1 NZ_CP081175:241234-241584 [Bacillus anthracis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T212638 NZ_CP107304:51026-51175 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT212638 WP_000004570.1 NZ_CP081175:240942-241229 [Bacillus anthracis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT212638 NZ_CP107304:c50981-50922 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References