Detailed information of TA system TA212622

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 1763951..1764165
• Replicon: chromosome
• Accession: NZ_CP081023
• Organism: Clostridioides difficile strain CD060

Toxin (Protein)

• Gene name: CD0956.2
• Uniprot ID: Q183Z5
• Locus tag: K2216_RS08310
• Protein ID: WP_003429855.1
• Coordinates: 1763951..1764112 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	RCd10
Locus tag	-
Coordinates	1764032..1764165 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K2216_RS08265 (1759291)	1759291..1759524	+	234	WP_003433536.1	hypothetical protein	-
K2216_RS08270 (1759534)	1759534..1759935	+	402	WP_009888836.1	hypothetical protein	-
K2216_RS08275 (1759929)	1759929..1760276	+	348	WP_003433540.1	hypothetical protein	-
K2216_RS08280 (1760276)	1760276..1760701	+	426	WP_003433542.1	HK97 gp10 family phage protein	-
K2216_RS08285 (1760694)	1760694..1761131	+	438	WP_003433544.1	hypothetical protein	-
K2216_RS08290 (1761124)	1761124..1761300	+	177	WP_003433548.1	hypothetical protein	-
K2216_RS08295 (1761301)	1761301..1762611	+	1311	WP_003433550.1	phage tail sheath family protein	-
K2216_RS08300 (1762628)	1762628..1763098	+	471	WP_003429851.1	phage tail tube protein	-
K2216_RS08305 (1763170)	1763170..1763610	+	441	WP_003429853.1	phage portal protein	-
K2216_RS08310 (1763951)	1763951..1764112	+	162	WP_003429855.1	hypothetical protein	Toxin
- (1764032)	1764032..1764165	-	134	NuclAT_0	-	Antitoxin
- (1764032)	1764032..1764165	-	134	NuclAT_0	-	Antitoxin
- (1764032)	1764032..1764165	-	134	NuclAT_0	-	Antitoxin
- (1764032)	1764032..1764165	-	134	NuclAT_1	-	Antitoxin
- (1764510)	1764510..1764558	-	49	NuclAT_4	-	-
- (1764510)	1764510..1764558	-	49	NuclAT_4	-	-
- (1764510)	1764510..1764558	-	49	NuclAT_5	-	-
- (1764510)	1764510..1764558	-	49	NuclAT_6	-	-
K2216_RS08315 (1765260)	1765260..1766012	+	753	WP_021387708.1	SHOCT domain-containing protein	-
K2216_RS08320 (1766316)	1766316..1766489	+	174	WP_003433557.1	hypothetical protein	-
K2216_RS08325 (1766613)	1766613..1767440	+	828	WP_003433559.1	hypothetical protein	-
K2216_RS08330 (1767489)	1767489..1767665	+	177	WP_003433561.1	hypothetical protein	-
K2216_RS08335 (1768096)	1768096..1768656	+	561	WP_021387709.1	ribosomal protein L7/L12	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6120.42 Da
• Isoelectric Point: 10.8938

>T212622 WP_003429855.1 NZ_CP081023:1763951-1764112 [Clostridioides difficile]
MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFDFKIKFHKFK

Nucleotide

• Length: 162 bp

>T212622 NZ_CP107298:169262-169732 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Nucleotide

• Length: 134 bp

>AT212622 NZ_CP081023:c1764165-1764032 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTTGCGGTAGAGTGAAGTTCATAATTAAATGAATCTATTTGAACTTATGGAACTTGATTTT
AAAATCAAATTCCCAGCCACTTTTACTCTTGCCACGAGTTGAGTGGCTTTTTAC

Structures

Toxin

• AlphaFold DB: A0A069AVN2

Antitoxin