Detailed information of TA system TA212588

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 1045372..1046057
• Replicon: chromosome
• Accession: NZ_CP081016
• Organism: Pseudomonas sp. 2hn

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: K2O50_RS04860
• Protein ID: WP_050704094.1
• Coordinates: 1045689..1046057 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: K2O50_RS04855
• Protein ID: WP_050704093.1
• Coordinates: 1045372..1045692 (-)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K2O50_RS04830 (K2O50_04830)	1041556..1041957	+	402	WP_050704088.1	hypothetical protein	-
K2O50_RS04835 (K2O50_04835)	1041950..1043326	+	1377	WP_050704089.1	class II fumarate hydratase	-
K2O50_RS04840 (K2O50_04840)	1043352..1043813	+	462	WP_050704090.1	DUF2753 family protein	-
K2O50_RS04845 (K2O50_04845)	1043815..1044432	+	618	WP_050704091.1	superoxide dismutase	-
K2O50_RS04850 (K2O50_04850)	1044438..1045331	+	894	WP_050704092.1	ZIP family metal transporter	-
K2O50_RS04855 (K2O50_04855)	1045372..1045692	-	321	WP_050704093.1	helix-turn-helix transcriptional regulator	Antitoxin
K2O50_RS04860 (K2O50_04860)	1045689..1046057	-	369	WP_050704094.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K2O50_RS04865 (K2O50_04865)	1046156..1046428	-	273	WP_050704095.1	HPr family phosphocarrier protein	-
K2O50_RS04870 (K2O50_04870)	1046449..1047303	-	855	WP_050704096.1	RNase adapter RapZ	-
K2O50_RS04875 (K2O50_04875)	1047306..1047770	-	465	WP_050704097.1	PTS IIA-like nitrogen regulatory protein PtsN	-
K2O50_RS04880 (K2O50_04880)	1047783..1048091	-	309	WP_011532415.1	ribosome-associated translation inhibitor RaiA	-
K2O50_RS04885 (K2O50_04885)	1048170..1049669	-	1500	WP_050704098.1	RNA polymerase factor sigma-54	-
K2O50_RS04890 (K2O50_04890)	1049842..1050567	-	726	WP_011532417.1	LPS export ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (18-111)

Antitoxin

• HigA2-like, helix-turn-helix domain (11-90)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13454.49 Da
• Isoelectric Point: 9.7833

>T212588 WP_050704094.1 NZ_CP081016:c1046057-1045689 [Pseudomonas sp. 2hn]
MESDRQPKPLYWVASSKKDLLCLPEDVQQTVGFALHLAQTGGRHLQAKVLRGFSGAGVLEVVEDHDGNAYRAVYTVRLGT
AVYVLHCFQKKSKAGIRTPETDIALVRKRLKLAQAHSEGIQA

Nucleotide

• Length: 369 bp

>T212588 NZ_CP107287:c27742-27494 [Escherichia coli]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 11710.74 Da
• Isoelectric Point: 10.0577

>AT212588 WP_050704093.1 NZ_CP081016:c1045692-1045372 [Pseudomonas sp. 2hn]
MSEIEQGSGNVYADLNYPDADEMLVKARLASKICSIIKARHLTQMQAAQILGLPQPKVSEMIRGKFRGISESKMIDCLAR
LGRDVQIVVKAAPRSRPQGRVEVIFA

Nucleotide

• Length: 321 bp

>AT212588 NZ_CP107287:c27497-27150 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA