Detailed information of TA system TA212527

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rnlAB/RnlA-RnlB
• Location: 2276128..2277565
• Replicon: chromosome
• Accession: NZ_CP081009
• Organism: Escherichia coli strain Stbl4

Toxin (Protein)

• Gene name: rnlA
• Uniprot ID: P52129
• Locus tag: K3830_RS10880
• Protein ID: WP_000155570.1
• Coordinates: 2276128..2277201 (+)
• Length: 358 a.a.

Antitoxin (Protein)

• Gene name: rnlB
• Uniprot ID: P52130
• Locus tag: K3830_RS10885
• Protein ID: WP_000461704.1
• Coordinates: 2277194..2277565 (+)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3830_RS10865 (2271561)	2271561..2273750	-	2190	WP_000136402.1	DEAD/DEAH box helicase	-
K3830_RS10870 (2273747)	2273747..2275363	-	1617	WP_000445380.1	anti-bacteriophage protein AbpA	-
K3830_RS10875 (2275723)	2275723..2275986	-	264	WP_000502050.1	DUF1524 domain-containing protein	-
K3830_RS10880 (2276128)	2276128..2277201	+	1074	WP_000155570.1	type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA	Toxin
K3830_RS10885 (2277194)	2277194..2277565	+	372	WP_000461704.1	type II toxin-antitoxin system antitoxin RnlB	Antitoxin
K3830_RS10890 (2277920)	2277920..2278783	+	864	WP_000787597.1	GTPase family protein	-
K3830_RS10895 (2278875)	2278875..2279696	+	822	WP_000197391.1	DUF932 domain-containing protein	-
K3830_RS10900 (2279913)	2279913..2280614	+	702	WP_000189409.1	WYL domain-containing protein	-
K3830_RS10905 (2280655)	2280655..2280891	+	237	WP_001144029.1	protein YpjK	-
K3830_RS10910 (2280891)	2280891..2281334	+	444	WP_000824222.1	lipoprotein YfjS	-
K3830_RS10915 (2281358)	2281358..2281825	+	468	WP_000702495.1	protein YfjT	-
K3830_RS22255 (2282212)	2282212..2282364	-	153	Protein_2146	arsenate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain (98-183)

Antitoxin

• Antitoxin RnlB/LsoB (14-111)

Sequences

Toxin

Protein

• Length: 358 a.a.
• Molecular weight: 40061.13 Da
• Isoelectric Point: 6.8293

>T212527 WP_000155570.1 NZ_CP081009:2276128-2277201 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Nucleotide

• Length: 1074 bp

>T212527 NZ_CP107257:789582-789863 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13664.69 Da
• Isoelectric Point: 4.2391

>AT212527 WP_000461704.1 NZ_CP081009:2277194-2277565 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Nucleotide

• Length: 372 bp

>AT212527 NZ_CP107257:789358-789585 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• PDB: 6Y2R
• PDB: 7AEX
• PDB: 6Y2Q
• PDB: 4I8O
• PDB: 6Y2P
• PDB: 6Y2S

Antitoxin

• PDB: 6Y2P
• AlphaFold DB: A0A373F4G4