Detailed information of TA system TA212511

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-ralA/-
• Location: 4371781..4372202
• Replicon: chromosome
• Accession: NZ_CP081008
• Organism: Escherichia coli strain XL10-gold-ultracompetent

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: K3831_RS21160
• Protein ID: WP_096937776.1
• Coordinates: 4371781..4371906 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	4372004..4372202 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3831_RS21130 (4366928)	4366928..4367491	-	564	WP_000052796.1	alkyl hydroperoxide reductase subunit C	-
K3831_RS21135 (4367863)	4367863..4368609	+	747	WP_000913829.1	thiol:disulfide interchange protein DsbG	-
K3831_RS21140 (4368818)	4368818..4369720	+	903	WP_000002474.1	LysR family transcriptional regulator	-
K3831_RS21145 (4369867)	4369867..4371111	+	1245	WP_220725813.1	phosphoadenosine phosphosulfate reductase	-
K3831_RS21150 (4371090)	4371090..4371230	-	141	Protein_4191	IS4 family transposase	-
K3831_RS23300 (4371173)	4371173..4371480	+	308	Protein_4192	hypothetical protein	-
K3831_RS21160 (4371781)	4371781..4371906	-	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
K3831_RS23305 (4371848)	4371848..4371997	-	150	Protein_4194	DUF5431 family protein	-
- (4372004)	4372004..4372202	-	199	NuclAT_1	-	Antitoxin
- (4372004)	4372004..4372202	-	199	NuclAT_1	-	Antitoxin
- (4372004)	4372004..4372202	-	199	NuclAT_1	-	Antitoxin
- (4372004)	4372004..4372202	-	199	NuclAT_1	-	Antitoxin
K3831_RS21165 (4372171)	4372171..4372933	-	763	Protein_4195	plasmid SOS inhibition protein A	-
K3831_RS21170 (4372930)	4372930..4373364	-	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
K3831_RS21175 (4373419)	4373419..4375377	-	1959	WP_000117173.1	ParB/RepB/Spo0J family partition protein	-
K3831_RS21180 (4375443)	4375443..4375676	-	234	WP_000005990.1	DUF905 family protein	-
K3831_RS21185 (4375733)	4375733..4376272	-	540	WP_000290801.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T212511 WP_096937776.1 NZ_CP081008:c4371906-4371781 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T212511 NZ_CP107241:4218816-4219073 [Xanthomonas hortorum]
GTGATTCTGCAGTTTGCTGACAATGCCTGGGAGGACTACCTCTACTGGCAACAGACCGACAAGAAGATGCTCAAACGCAT
CAACGAGTTGATCAAGGCCATTCAGCGCGATCCTTTCCAGGGCATCGGCAAACCCGAACCGTTGCGCCACGCGCTGGCAG
GCTATTGGTCACGACGCATCAATGACGAACACCGGGTTGTCTACAAGGCCGAGAACGGCATCCTGCTGATTGCTCAGGCG
CGCTACCACTACGCGTAA

Antitoxin

Nucleotide

• Length: 199 bp

>AT212511 NZ_CP081008:c4372202-4372004 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin