Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1918711..1919325 Replicon chromosome
Accession NZ_CP080511
Organism Pseudomonas aeruginosa strain DJ06

Toxin (Protein)


Gene name hicA Uniprot ID A6VBH9
Locus tag K1T67_RS08795 Protein ID WP_071534354.1
Coordinates 1918711..1918893 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A140SDX7
Locus tag K1T67_RS08800 Protein ID WP_012077229.1
Coordinates 1918921..1919325 (+) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K1T67_RS08770 (K1T67_08770) 1914352..1914747 + 396 WP_003094374.1 hypothetical protein -
K1T67_RS08775 (K1T67_08775) 1914740..1916116 + 1377 WP_003098863.1 class II fumarate hydratase FumC -
K1T67_RS08780 (K1T67_08780) 1916144..1916593 + 450 WP_003094369.1 hypothetical protein -
K1T67_RS08785 (K1T67_08785) 1916606..1917217 + 612 WP_003098862.1 superoxide dismutase -
K1T67_RS08790 (K1T67_08790) 1917236..1918168 + 933 WP_003120918.1 ZIP family metal transporter -
K1T67_RS08795 (K1T67_08795) 1918711..1918893 + 183 WP_071534354.1 type II toxin-antitoxin system HicA family toxin Toxin
K1T67_RS08800 (K1T67_08800) 1918921..1919325 + 405 WP_012077229.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
K1T67_RS08805 (K1T67_08805) 1919368..1920339 - 972 WP_012077228.1 hypothetical protein -
K1T67_RS08810 (K1T67_08810) 1920324..1921022 - 699 WP_033896049.1 hypothetical protein -
K1T67_RS08815 (K1T67_08815) 1921022..1921561 - 540 WP_012074127.1 hypothetical protein -
K1T67_RS08820 (K1T67_08820) 1921558..1922478 - 921 WP_012074128.1 hypothetical protein -
K1T67_RS08825 (K1T67_08825) 1922475..1923185 - 711 WP_012074129.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1918711..1956205 37494


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6702.74 Da        Isoelectric Point: 10.4826

>T211965 WP_071534354.1 NZ_CP080511:1918711-1918893 [Pseudomonas aeruginosa]
MRSREVIDLLLEDGWYEVAVKGSHHQFKHPSKPGKVTVQHPSSTIPKGTLNNILKQAGLK

Download         Length: 183 bp

>T211965 NZ_CP106886:4180534-4180752 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14670.54 Da        Isoelectric Point: 4.4315

>AT211965 WP_012077229.1 NZ_CP080511:1918921-1919325 [Pseudomonas aeruginosa]
MKFPVVLHKDPDSDYGVTVPDVPGCFSAGATVSEALANVEEALALHFEGLVTDGEELPQPQDVDAHMKNPDFEGGVWAVV
DFDVTPYLGKAVRFNATLPEHLLQRIDERVKVDKRYQSRSGFLATAAMRELSVA

Download         Length: 405 bp

>AT211965 NZ_CP106886:4180133-4180507 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A6VBH9

Antitoxin

Source ID Structure
AlphaFold DB A0A140SDX7

References