Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 628894..629693 Replicon chromosome
Accession NZ_CP080255
Organism Escherichia coli strain SJC33

Toxin (Protein)


Gene name yhaV Uniprot ID F0JQM9
Locus tag KZ313_RS03005 Protein ID WP_000347270.1
Coordinates 628894..629358 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A0V9H1L4
Locus tag KZ313_RS03010 Protein ID WP_001551693.1
Coordinates 629358..629693 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZ313_RS02975 (623895) 623895..624329 - 435 WP_000948837.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KZ313_RS02980 (624347) 624347..625225 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
KZ313_RS02985 (625215) 625215..625994 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KZ313_RS02990 (626005) 626005..626478 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KZ313_RS02995 (626501) 626501..627781 - 1281 WP_001551694.1 tagatose-bisphosphate aldolase subunit KbaZ -
KZ313_RS03000 (628030) 628030..628839 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KZ313_RS03005 (628894) 628894..629358 - 465 WP_000347270.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KZ313_RS03010 (629358) 629358..629693 - 336 WP_001551693.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KZ313_RS03015 (629842) 629842..631413 - 1572 WP_069903725.1 galactarate dehydratase -
KZ313_RS03020 (631788) 631788..633122 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
KZ313_RS03025 (633138) 633138..633908 + 771 WP_001551692.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T211476 WP_000347270.1 NZ_CP080255:c629358-628894 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Download         Length: 465 bp

>T211476 NZ_CP104869:c4648060-4647305 [Pseudomonas aeruginosa]
GTGAGCGAGATCTGGCGACAGTGCAAGGGTGAGCGGCATGTCCGACCGCTCCAGGGGCGGCTGGTGCGGTTGGTGGAGAG
CCAGGGGCAGGTGGCGACCCTGCAATTGGTCGACACCCTGGAAGAACAGGCGTTGCTCGAGGAACTGCTCGAGTCGAGCA
AGCCGCCGGTGCCGGCGGATGCCGAGCCATTGCATTACCTGTTGAAGACCCCGTTCCGTTATCCGCCCTTGCGTTGGGGG
TCGCGGTTTGGCCGTCGTCACGAGCCAAGCCTGTTCTACGCCGCGTTGAAGCTGGAAACGGCCATGGCCGAAAGCGCGTA
TTACCGTTGTGTCCTGTGGAGTGGCATGGCGGTGCCGCCACCGAGCGGGAGGATTCTGTCCGAGCACGCTTCGTTCGAGG
CGGGCTGGAAGGTGGAGCGGGGAATCCGTCTGCAGGCGCCGCCTTTCTCCGATCACGAGGCTGCGCTGACCGACATCGCC
GACTACCGCGCTCCCCAGGAACTGGGGAGTGCCATGCGCAGCGCCGGGGTGCAGGCATTCGAATACCGTTCGGCGCGTTG
CCCGGAGCGCGGCTGCAATGTCGCCCTGTTCACGCCCGCGGCCTTCACCGAAAAGCGTCCTCGCAACCTGACGCCCTGGC
TGTGCGAAACCACGGCCGGTTACGTGGCCTTCAAGCCGGCGCATGTGCCGGGGTCGCCGAAAATCTTCTCCTGGGAGCTG
TTCCTGGTGGACGGAAAGTTGCCGCATCCGGCGTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12347.87 Da        Isoelectric Point: 4.6890

>AT211476 WP_001551693.1 NZ_CP080255:c629693-629358 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT211476 NZ_CP104869:c4648563-4648195 [Pseudomonas aeruginosa]
ATGAGCGCTCTCATCAAGGAACGTCCCAGCGCCGATGCTGTCCTGGCCAAGGCCGTCCTGGCCGCGCGCGAGCAATTGGG
GCTGACGCAGCTCGAACTGGCCGGCATCGTCGGCGTCGATCGCAGCGCCATCAGCCGCTGGAAGACCCAGGGCCTGCGGG
TGGACAGCAAGACCGGCGAGCTGGCTCTGCTGCTGGTGCGAGTCTATCGCGCACTGTATGCCCTGTTCGGCGGGCAGCAG
GAGGACATGCGCCACTTCCTGCGCACTCCCAACCATCACCTGGCGGGCGAGCCGCTGGCACTGATGGGACAGGTGCAGGG
CCTGGTTCATGTGCTGGAATACCTCGATGCGATCCGCGGCAAGGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9NQ90

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9H1L4

References