Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4380194..4380992 Replicon chromosome
Accession NZ_CP080246
Organism Escherichia coli strain SYNB1934

Toxin (Protein)


Gene name yeeV Uniprot ID Q8VRA6
Locus tag KZX95_RS21040 Protein ID WP_000854730.1
Coordinates 4380194..4380571 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0P0SQV8
Locus tag KZX95_RS21045 Protein ID WP_001285481.1
Coordinates 4380618..4380992 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX95_RS21015 (4375338) 4375338..4376742 + 1405 Protein_4117 IS66 family transposase -
KZX95_RS21020 (4376996) 4376996..4378129 - 1134 WP_000555401.1 IS110-like element ISEc45 family transposase -
KZX95_RS24250 (4378190) 4378190..4378312 + 123 Protein_4119 transposase domain-containing protein -
KZX95_RS24255 (4378302) 4378302..4378490 + 189 Protein_4120 IS66 family transposase -
KZX95_RS21030 (4378638) 4378638..4379626 + 989 Protein_4121 IS630 family transposase -
KZX95_RS21035 (4379705) 4379705..4380197 - 493 Protein_4122 DUF5983 family protein -
KZX95_RS21040 (4380194) 4380194..4380571 - 378 WP_000854730.1 TA system toxin CbtA family protein Toxin
KZX95_RS21045 (4380618) 4380618..4380992 - 375 WP_001285481.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
KZX95_RS21050 (4381042) 4381042..4381686 - 645 WP_000086759.1 hypothetical protein -
KZX95_RS21055 (4381705) 4381705..4381926 - 222 WP_000692315.1 DUF987 domain-containing protein -
KZX95_RS21060 (4381989) 4381989..4382465 - 477 WP_001313574.1 RadC family protein -
KZX95_RS21065 (4382481) 4382481..4382954 - 474 WP_001313575.1 antirestriction protein -
KZX95_RS21070 (4383048) 4383048..4383293 - 246 WP_001164966.1 hypothetical protein -
KZX95_RS21075 (4383293) 4383293..4384114 - 822 WP_024199417.1 DUF932 domain-containing protein -
KZX95_RS21080 (4384214) 4384214..4384447 - 234 WP_001213776.1 DUF905 family protein -
KZX95_RS21085 (4384565) 4384565..4385017 - 453 WP_000682723.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14212.21 Da        Isoelectric Point: 7.2923

>T211373 WP_000854730.1 NZ_CP080246:c4380571-4380194 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRSNYRMVNDIIRGEHSEAKR

Download         Length: 378 bp

>T211373 NZ_CP104848:c3317909-3317658 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13817.53 Da        Isoelectric Point: 4.7511

>AT211373 WP_001285481.1 NZ_CP080246:c4380992-4380618 [Escherichia coli]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTIG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Download         Length: 375 bp

>AT211373 NZ_CP104848:c3317661-3317407 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H2V671

Antitoxin

Source ID Structure
AlphaFold DB A0A0P0SQV8

References