Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 991110..991764 Replicon chromosome
Accession NZ_CP080246
Organism Escherichia coli strain SYNB1934

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag KZX95_RS04855 Protein ID WP_000244781.1
Coordinates 991357..991764 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KZX95_RS04850 Protein ID WP_000354046.1
Coordinates 991110..991376 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX95_RS04830 987198..988631 - 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -
KZX95_RS04835 988676..988987 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
KZX95_RS04840 989151..989810 + 660 WP_000250274.1 hemolysin III family protein -
KZX95_RS04845 989887..990867 - 981 WP_000886079.1 tRNA-modifying protein YgfZ -
KZX95_RS04850 991110..991376 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KZX95_RS04855 991357..991764 + 408 WP_000244781.1 protein YgfX Toxin
KZX95_RS04860 991804..992325 - 522 WP_001055874.1 flavodoxin FldB -
KZX95_RS04865 992437..993333 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KZX95_RS04870 993358..994068 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KZX95_RS04875 994074..995807 + 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T211351 WP_000244781.1 NZ_CP080246:991357-991764 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T211351 NZ_CP104846:c4735288-4734977 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT211351 WP_000354046.1 NZ_CP080246:991110-991376 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT211351 NZ_CP104846:c4734976-4734686 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References