Detailed information of TA system TA211350

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 849911..850745
• Replicon: chromosome
• Accession: NZ_CP080246
• Organism: Escherichia coli strain SYNB1934

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PLF5
• Locus tag: KZX95_RS04110
• Protein ID: WP_000854690.1
• Coordinates: 849911..850288 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: KZX95_RS04115
• Protein ID: WP_119023792.1
• Coordinates: 850377..850745 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZX95_RS04080 (846305)	846305..846475	-	171	Protein_802	IS110 family transposase	-
KZX95_RS04085 (846892)	846892..847825	-	934	Protein_803	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
KZX95_RS04090 (847818)	847818..848213	-	396	WP_000208382.1	DUF6088 family protein	-
KZX95_RS04095 (848282)	848282..849127	-	846	WP_001529401.1	DUF4942 domain-containing protein	-
KZX95_RS04100 (849212)	849212..849409	-	198	WP_000839293.1	DUF957 domain-containing protein	-
KZX95_RS04105 (849426)	849426..849914	-	489	WP_000761699.1	DUF5983 family protein	-
KZX95_RS04110 (849911)	849911..850288	-	378	WP_000854690.1	TA system toxin CbtA family protein	Toxin
KZX95_RS04115 (850377)	850377..850745	-	369	WP_119023792.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
KZX95_RS04120 (850795)	850795..851438	-	644	Protein_810	antitoxin of toxin-antitoxin stability system	-
KZX95_RS04125 (851457)	851457..851678	-	222	WP_000692329.1	DUF987 domain-containing protein	-
KZX95_RS04130 (851741)	851741..852217	-	477	WP_001186726.1	RadC family protein	-
KZX95_RS04135 (852233)	852233..852718	-	486	WP_000849565.1	antirestriction protein	-
KZX95_RS04140 (852773)	852773..853591	-	819	WP_001234620.1	DUF932 domain-containing protein	-
KZX95_RS04145 (853692)	853692..853925	-	234	WP_000902034.1	DUF905 family protein	-
KZX95_RS04150 (854004)	854004..854459	-	456	WP_000581502.1	IrmA family protein	-
KZX95_RS04155 (854535)	854535..855662	-	1128	Protein_817	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	ugd / kpsS / kpsC / kpsU / kpsD / kpsE / kpsF / sat	830361..897678	67317

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14057.04 Da
• Isoelectric Point: 9.1510

>T211350 WP_000854690.1 NZ_CP080246:c850288-849911 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYAQVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T211350 NZ_CP104846:c4418938-4418765 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTTTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13601.51 Da
• Isoelectric Point: 4.5851

>AT211350 WP_119023792.1 NZ_CP080246:c850745-850377 [Escherichia coli]
MSDTLPGTTLPDDNKDLPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT211350 NZ_CP104846:c4419262-4418966 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: S1PLF5