Detailed information of TA system TA211349

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 629064..629863
• Replicon: chromosome
• Accession: NZ_CP080246
• Organism: Escherichia coli strain SYNB1934

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: Q8FDB4
• Locus tag: KZX95_RS03070
• Protein ID: WP_000347252.1
• Coordinates: 629064..629528 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1PPV5
• Locus tag: KZX95_RS03075
• Protein ID: WP_001296435.1
• Coordinates: 629528..629863 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZX95_RS03040 (624065)	624065..624499	-	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
KZX95_RS03045 (624517)	624517..625395	-	879	WP_001298314.1	PTS N-acetylgalactosamine transporter subunit IID	-
KZX95_RS03050 (625385)	625385..626164	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
KZX95_RS03055 (626175)	626175..626648	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
KZX95_RS03060 (626671)	626671..627951	-	1281	WP_000681926.1	tagatose-bisphosphate aldolase subunit KbaZ	-
KZX95_RS03065 (628200)	628200..629009	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
KZX95_RS03070 (629064)	629064..629528	-	465	WP_000347252.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
KZX95_RS03075 (629528)	629528..629863	-	336	WP_001296435.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
KZX95_RS03080 (630012)	630012..631583	-	1572	WP_001273940.1	galactarate dehydratase	-
KZX95_RS03085 (631958)	631958..633292	+	1335	WP_000979025.1	galactarate/glucarate/glycerate transporter GarP	-
KZX95_RS03090 (633308)	633308..634078	+	771	WP_001058223.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17750.11 Da
• Isoelectric Point: 9.4947

>T211349 WP_000347252.1 NZ_CP080246:c629528-629064 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEESH

Nucleotide

• Length: 465 bp

>T211349 NZ_CP104846:c4316014-4315664 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12321.92 Da
• Isoelectric Point: 4.5392

>AT211349 WP_001296435.1 NZ_CP080246:c629863-629528 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT211349 NZ_CP104846:c4316259-4316008 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: Q8FDB4

Antitoxin

• AlphaFold DB: S1PPV5