Detailed information of TA system TA211348

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 50620..51411
• Replicon: chromosome
• Accession: NZ_CP080246
• Organism: Escherichia coli strain SYNB1934

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: KZX95_RS00245
• Protein ID: WP_000691791.1
• Coordinates: 50620..51045 (-)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A3L1KVZ6
• Locus tag: KZX95_RS00250
• Protein ID: WP_000939437.1
• Coordinates: 51076..51411 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZX95_RS00220 (46286)	46286..46783	-	498	WP_000509808.1	hypothetical protein	-
KZX95_RS00225 (47465)	47465..47851	-	387	WP_001270938.1	DUF2441 domain-containing protein	-
KZX95_RS00230 (48474)	48474..49205	-	732	WP_001216639.1	retron system putative HNH endonuclease	-
KZX95_RS00235 (49202)	49202..49423	-	222	WP_125112077.1	hypothetical protein	-
KZX95_RS00240 (49408)	49408..50599	-	1192	Protein_47	AAA family ATPase	-
KZX95_RS00245 (50620)	50620..51045	-	426	WP_000691791.1	TA system toxin CbtA family protein	Toxin
KZX95_RS00250 (51076)	51076..51411	-	336	WP_000939437.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KZX95_RS00255 (51411)	51411..51884	-	474	WP_001292742.1	DNA repair protein RadC	-
KZX95_RS00260 (51914)	51914..52728	-	815	Protein_51	DUF945 domain-containing protein	-
KZX95_RS00265 (52964)	52964..53917	-	954	WP_000290406.1	hypothetical protein	-
KZX95_RS00270 (54510)	54510..55124	+	615	WP_000772910.1	inovirus Gp2 family protein	-
KZX95_RS00275 (55242)	55242..55460	+	219	WP_000070772.1	AlpA family phage regulatory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	48474..72405	23931

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (10-110)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 16490.11 Da
• Isoelectric Point: 10.2376

>T211348 WP_000691791.1 NZ_CP080246:c51045-50620 [Escherichia coli]
MKIIPATTLRATTSYLSPVVVWQTLLARLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVNAVNFLVEKYELVRIDRKGF
SWQEQTPYLRAVDILRARQATGLRIQCKNTNIISRMADSRHDKRLYQRTFKACKRCYYTKT

Nucleotide

• Length: 426 bp

>T211348 NZ_CP104846:c4121190-4120783 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12556.56 Da
• Isoelectric Point: 7.6977

>AT211348 WP_000939437.1 NZ_CP080246:c51411-51076 [Escherichia coli]
MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADGELSPCQQR
TVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT211348 NZ_CP104846:c4121437-4121171 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCACCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A3L1KVZ6