Detailed information of TA system TA211309

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 544500..544871
• Replicon: chromosome
• Accession: NZ_CP080235
• Organism: Escherichia coli O139:H1 strain W13-16

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A839BBC2
• Locus tag: KZW92_RS02810
• Protein ID: WP_021500490.1
• Coordinates: 544677..544871 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	544500..544678 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZW92_RS02785 (540455)	540455..541828	+	1374	WP_063085924.1	ATP-dependent RNA helicase DbpA	-
KZW92_RS02790 (541957)	541957..542892	-	936	WP_001157382.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
KZW92_RS02795 (542944)	542944..544179	-	1236	WP_024168543.1	site-specific integrase	-
KZW92_RS02800 (544181)	544181..544396	-	216	WP_000079604.1	excisionase XisR	-
- (544500)	544500..544678	+	179	NuclAT_1	-	Antitoxin
- (544500)	544500..544678	+	179	NuclAT_1	-	Antitoxin
- (544500)	544500..544678	+	179	NuclAT_1	-	Antitoxin
- (544500)	544500..544678	+	179	NuclAT_1	-	Antitoxin
KZW92_RS02805 (544496)	544496..544684	-	189	WP_001372676.1	DUF1187 family protein	-
KZW92_RS02810 (544677)	544677..544871	-	195	WP_021500490.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
KZW92_RS02815 (544935)	544935..545987	-	1053	WP_001004412.1	RecT family recombinase	-
KZW92_RS02820 (545999)	545999..549121	-	3123	WP_219789599.1	exodeoxyribonuclease VIII	-
KZW92_RS02825 (549223)	549223..549498	-	276	WP_001351479.1	hypothetical protein	-
KZW92_RS02830 (549573)	549573..549737	-	165	WP_236265422.1	Rtr1/RPAP2 family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	542944..589693	46749

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7046.98 Da
• Isoelectric Point: 9.2886

>T211309 WP_021500490.1 NZ_CP080235:c544871-544677 [Escherichia coli O139:H1]
MRYEKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T211309 NZ_CP104843:c836541-836164 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGTACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT211309 NZ_CP080235:544500-544678 [Escherichia coli O139:H1]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTCCCTGA
GAGCATTTTTTCGCATTCCGATTTGGTTAACTTTGTTTTTGAGTACCTTGTCCAGCTGGTAGGAGAACCACCTTCCTTTT
CAATAGTGGCGGTGATTTT

Structures

Toxin

• AlphaFold DB: A0A839BBC2

Antitoxin