Detailed information of TA system TA211288

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2038147..2038981
• Replicon: chromosome
• Accession: NZ_CP080233
• Organism: Escherichia coli O139:H1 strain P15-25

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A3S7R4W2
• Locus tag: KZW90_RS09775
• Protein ID: WP_063085447.1
• Coordinates: 2038604..2038981 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A2J4HRK6
• Locus tag: KZW90_RS09770
• Protein ID: WP_001354276.1
• Coordinates: 2038147..2038515 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZW90_RS09745 (2033688)	2033688..2035244	-	1557	WP_086186061.1	type I restriction-modification system subunit M	-
KZW90_RS09750 (2035508)	2035508..2036380	+	873	WP_053271975.1	GTPase family protein	-
KZW90_RS09755 (2036753)	2036753..2037625	+	873	Protein_1903	autotransporter adhesin family protein	-
KZW90_RS09760 (2037594)	2037594..2037890	+	297	Protein_1904	antirestriction protein	-
KZW90_RS09770 (2038147)	2038147..2038515	+	369	WP_001354276.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
KZW90_RS09775 (2038604)	2038604..2038981	+	378	WP_063085447.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
KZW90_RS09780 (2038978)	2038978..2039106	+	129	Protein_1907	DUF5983 family protein	-
KZW90_RS09785 (2039086)	2039086..2039328	+	243	WP_242378885.1	DUF4942 domain-containing protein	-
KZW90_RS09795 (2039799)	2039799..2040737	+	939	WP_001357083.1	glyoxylate/hydroxypyruvate reductase GhrA	-
KZW90_RS09800 (2040792)	2040792..2041529	+	738	WP_000283664.1	zinc-binding phosphatase	-
KZW90_RS09805 (2041553)	2041553..2042107	+	555	WP_063085449.1	molecular chaperone YcdY	-
KZW90_RS09810 (2042209)	2042209..2042700	+	492	WP_032140678.1	DUF1097 domain-containing protein	-
KZW90_RS09815 (2042765)	2042765..2043598	-	834	WP_001189321.1	curli production assembly/transport protein CsgG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13908.94 Da
• Isoelectric Point: 8.5222

>T211288 WP_063085447.1 NZ_CP080233:2038604-2038981 [Escherichia coli O139:H1]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNNTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T211288 NZ_CP104835:c5320030-5319749 [Achromobacter xylosoxidans]
GTGCCCGCAGTTGAATGGTCGTCCGCCGCCCGGGCCGACCTGTTGGCCATAGTCGACTACATATCAGATGACAATCCAGA
CGCTGCCCAACGTCTGAAAGACGATATTGAAACGAAGGCGGCGCAACTGCCCGCCCGTCCCGCGCTGTACCGCCCCGGAA
GGATCGCCGGTACCCGCGAAATGGTCGTTCGCGCGAATTACATCATCGTGTATGCGGCGGATGCGCTGGCCGTGCGGATC
CTGCGCATCTTGCATGCCGCGCAGCAATGGCCGCCGCAGTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13649.47 Da
• Isoelectric Point: 7.0261

>AT211288 WP_001354276.1 NZ_CP080233:2038147-2038515 [Escherichia coli O139:H1]
VSDTLSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCKADTLSSCGYVYLAVYPTPKMKN

Nucleotide

• Length: 369 bp

>AT211288 NZ_CP104835:c5320313-5320017 [Achromobacter xylosoxidans]
ATGCCCCAGACCTCGATGCTCCACGTCCGCGTGGACGACAAGCTCAAAGCCGACGCCACCGAAAAGCTGGCCGAATTCGG
CCTGACGATTTCCGACGCCGTGCGCATCCTGCTCACGCGGGTGGCCAAGGAGGGCGGCCTGCCCGCCGGGTTGACCACCG
ATGCCCAGGCGCACGACGCGTGGTTCCGCGCCAAAGTCCAGGAAGCCCTGGCCGATACGGGGCCGGCGGTGCCGCACCAG
CAGGTCATGGATGAAGCCCAGGCGCTGATCGACAGGAAGCGCCGTGCCCGCAGTTGA

Structures

Toxin

• AlphaFold DB: A0A3S7R4W2

Antitoxin

• AlphaFold DB: A0A2J4HRK6