Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2937623..2938277 Replicon chromosome
Accession NZ_CP080139
Organism Escherichia coli strain PK8241

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag KZY05_RS15025 Protein ID WP_000244777.1
Coordinates 2937623..2938030 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KZY05_RS15030 Protein ID WP_000354046.1
Coordinates 2938011..2938277 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY05_RS15005 (2933580) 2933580..2935313 - 1734 WP_000813208.1 single-stranded-DNA-specific exonuclease RecJ -
KZY05_RS15010 (2935319) 2935319..2936029 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KZY05_RS15015 (2936054) 2936054..2936950 - 897 WP_032202431.1 site-specific tyrosine recombinase XerD -
KZY05_RS15020 (2937062) 2937062..2937583 + 522 WP_001055874.1 flavodoxin FldB -
KZY05_RS15025 (2937623) 2937623..2938030 - 408 WP_000244777.1 protein YgfX Toxin
KZY05_RS15030 (2938011) 2938011..2938277 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KZY05_RS15035 (2938520) 2938520..2939500 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KZY05_RS15040 (2939696) 2939696..2940355 - 660 WP_000250274.1 hemolysin III family protein -
KZY05_RS15045 (2940519) 2940519..2940830 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
KZY05_RS15050 (2940875) 2940875..2942308 + 1434 WP_069722973.1 6-phospho-beta-glucosidase BglA -
KZY05_RS15055 (2942365) 2942365..2943108 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T211033 WP_000244777.1 NZ_CP080139:c2938030-2937623 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T211033 NZ_CP104665:3030239-3030733 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT211033 WP_000354046.1 NZ_CP080139:c2938277-2938011 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT211033 NZ_CP104665:3029949-3030242 [Escherichia coli]
ATGGCAGCAGCCAATAACATTCCAGCCAGGCGCGAATCTTTAAACTTGCGCATAAAACCCGCAGAACGCGATCTTATAGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCTCCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References