Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 78243..78868 Replicon plasmid pPK8568-tetX
Accession NZ_CP080129
Organism Escherichia coli strain PK8568

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag KZY03_RS24535 Protein ID WP_000911324.1
Coordinates 78243..78641 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID L4J1D2
Locus tag KZY03_RS24540 Protein ID WP_000450532.1
Coordinates 78641..78868 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY03_RS24520 (74529) 74529..75038 + 510 WP_031943494.1 conjugal transfer entry exclusion protein TraS -
KZY03_RS24525 (75052) 75052..75783 + 732 WP_000850424.1 conjugal transfer complement resistance protein TraT -
KZY03_RS24530 (76036) 76036..78234 + 2199 WP_001496532.1 type IV conjugative transfer system coupling protein TraD -
KZY03_RS24535 (78243) 78243..78641 - 399 WP_000911324.1 type II toxin-antitoxin system VapC family toxin Toxin
KZY03_RS24540 (78641) 78641..78868 - 228 WP_000450532.1 toxin-antitoxin system antitoxin VapB Antitoxin

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(X4) / floR / blaTEM-1C - 1..91844 91844


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14800.09 Da        Isoelectric Point: 7.8604

>T210987 WP_000911324.1 NZ_CP080129:c78641-78243 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMEVIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELALQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Download         Length: 399 bp

>T210987 NZ_CP104649:5457489-5457644 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8512.55 Da        Isoelectric Point: 4.5358

>AT210987 WP_000450532.1 NZ_CP080129:c78868-78641 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Download         Length: 228 bp

>AT210987 NZ_CP104649:c5457477-5457419 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A829CHF5

References