Detailed information of TA system TA210130

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 4243392..4243989
• Replicon: chromosome
• Accession: NZ_CP079802
• Organism: Klebsiella variicola strain EMBL-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0B7GEF2
• Locus tag: KU883_RS20585
• Protein ID: WP_012542526.1
• Coordinates: 4243672..4243989 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KU883_RS20580
• Protein ID: WP_012542525.1
• Coordinates: 4243392..4243679 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU883_RS20550 (KU883_20540)	4239302..4239550	+	249	WP_008805539.1	DUF1158 domain-containing protein	-
KU883_RS20555 (KU883_20545)	4239567..4239908	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
KU883_RS20560 (KU883_20550)	4239939..4241054	-	1116	WP_162493283.1	MBL fold metallo-hydrolase	-
KU883_RS20565 (KU883_20555)	4241234..4241815	+	582	WP_012968754.1	TetR/AcrR family transcriptional regulator	-
KU883_RS20570 (KU883_20560)	4241815..4242183	+	369	WP_008805536.1	MmcQ/YjbR family DNA-binding protein	-
KU883_RS20575 (KU883_20565)	4242303..4242956	+	654	WP_012968755.1	oxygen-insensitive NAD(P)H nitroreductase	-
KU883_RS20580 (KU883_20570)	4243392..4243679	-	288	WP_012542525.1	helix-turn-helix transcriptional regulator	Antitoxin
KU883_RS20585 (KU883_20575)	4243672..4243989	-	318	WP_012542526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KU883_RS20590 (KU883_20580)	4244174..4245217	-	1044	WP_016160437.1	DUF2157 domain-containing protein	-
KU883_RS20595 (KU883_20585)	4245747..4246613	-	867	WP_008805530.1	helix-turn-helix transcriptional regulator	-
KU883_RS20600 (KU883_20590)	4246722..4248149	+	1428	WP_023297179.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-89)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12110.39 Da
• Isoelectric Point: 11.2767

>T210130 WP_012542526.1 NZ_CP079802:c4243989-4243672 [Klebsiella variicola]
MFRLVVHVDVKKELQALPPIVQAKMIRQIDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T210130 NZ_CP104405:1773333-1773854 [Enterobacter hormaechei subsp. steigerwaltii]
GTGGCAAATCTCACGATCGAAATGTTATCAGAGGGAACCGATTACGATTTCGGCGATTTCGATTGTGGTGAACCCTCGCT
AAATGCCTTCCTTACCGAGCACCTGATACGTCAGCACGGCGGGCGTATTTTACGCGGCTACCTGCTTAAAGAACGCGACC
ATCCGCGTGTCCTGGGCTATTACACACTCTCGGGAAGTTGCTTTGAAAAAGTGATGCTTCCTTCAAAGACTCAGCAGCGC
CGCATCCCATACAGCAACGTCCCCAGCGTGACGCTGGGGCGTCTGGCTGTCCATAAAGAACTCCAGGGAAACGAGTGGGG
AACAACGCTTGTCACACACGCTATGCGCGTTGTTTATCTGGCCTCGCAGGCTGTTGGCGTCCACGGAATTTTTGTGGATG
CGCTTAACGAACGGGCCAAACGATTTTATCTGAAGCTGGGGTTTATCCCGCTGGCAGGTGAAAACAGCAGTTCGCTGTTC
TTCCCCACGCAATCTATAGAACGACTGTTTGAGCAGGCGTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10815.51 Da
• Isoelectric Point: 10.2212

>AT210130 WP_012542525.1 NZ_CP079802:c4243679-4243392 [Klebsiella variicola]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQILLY

Nucleotide

• Length: 288 bp

>AT210130 NZ_CP104405:1773057-1773326 [Enterobacter hormaechei subsp. steigerwaltii]
ATGCCAGCACTCAAAAAACAGCGTATCGATCTTCGCCTGTCGGACGAAGACAAAACAATGATTGAAGAAGCGGCAGCGAT
GACCAACCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGTGCTGCCGAAGTCATCGAACAGCATCGGCGTC
TCGTATTAAACGAAGCCTCATGGATTGCCGTTATGGATGCCATCGACAATCCGCCAGAGCCAAATGAACGCCTGAAGCGA
GCGGCGAAACGTCTTCGAAACATGGAGTGA

Structures

Toxin

• AlphaFold DB: A0A0B7GEF2