Detailed information of TA system TA209924

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 3920232..3921927
• Replicon: chromosome
• Accession: NZ_CP079757
• Organism: Pseudomonas aeruginosa strain PA0200

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9HYA0
• Locus tag: KXJ64_RS18215
• Protein ID: WP_003112901.1
• Coordinates: 3921058..3921927 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: Q9HYA1
• Locus tag: KXJ64_RS18210
• Protein ID: WP_003112902.1
• Coordinates: 3920232..3921065 (-)
• Length: 278 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KXJ64_RS18190 (KXJ64_18125)	3915463..3916947	-	1485	WP_003112906.1	aldehyde dehydrogenase	-
KXJ64_RS18195 (KXJ64_18130)	3916959..3917762	-	804	WP_003112905.1	aspartate dehydrogenase	-
KXJ64_RS18200 (KXJ64_18135)	3917773..3919455	-	1683	WP_003112904.1	thiamine pyrophosphate-binding protein	-
KXJ64_RS18205 (KXJ64_18140)	3919448..3920245	-	798	WP_003112903.1	SDR family oxidoreductase	-
KXJ64_RS18210 (KXJ64_18145)	3920232..3921065	-	834	WP_003112902.1	IclR family transcriptional regulator	Antitoxin
KXJ64_RS18215 (KXJ64_18150)	3921058..3921927	-	870	WP_003112901.1	alpha/beta hydrolase	Toxin
KXJ64_RS18220 (KXJ64_18155)	3921936..3922466	-	531	WP_003112900.1	cupin domain-containing protein	-
KXJ64_RS18225 (KXJ64_18160)	3922480..3923241	-	762	WP_003112899.1	SDR family oxidoreductase	-
KXJ64_RS18230 (KXJ64_18165)	3923247..3924023	-	777	WP_003112898.1	ABC transporter permease	-
KXJ64_RS18235 (KXJ64_18170)	3924020..3925027	-	1008	WP_003112897.1	ABC transporter substrate-binding protein	-
KXJ64_RS18240 (KXJ64_18175)	3925068..3925919	-	852	WP_003130104.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (41-272)

Antitoxin

• Transcription regulator IclR, N-terminal (16-66)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 30403.60 Da
• Isoelectric Point: 6.5894

>T209924 WP_003112901.1 NZ_CP079757:c3921927-3921058 [Pseudomonas aeruginosa]
MNMAQTHTPVPNLQQRFPERLVQLADGAQLAIRECGQGPVVVLLHGIGSGSASWLHCAQRLAAGNRVIAWDAPGYGLSTP
LPPARPKACDYAACLELLLDALGVESCLLVGHSLGALMATAYAAGIGAARVRRLVLLSPARGYGAAELRDSGAQVRRQRL
ENLERFGIDGMASERTARLLGRNPSEEALAWVRWNMARLNPEGYRQAVELLCGDDLLGNGQPAAPCEVHCGEDDGITTPE
SCGAIARQLGASFSSIPGAGHASPIEQPEVVAGRIGHAQRLSLEGTANG

Nucleotide

• Length: 870 bp

>T209924 NZ_CP104340:c1590754-1590572 [Acinetobacter baumannii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGATGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
TAAACACCCAACCAAAAAAGGGTTAGTTACAATCTCACATCCTAAAAAGGATTTACCAAACGGAACTGTTAAAAGCATTT
TGAAACAAGCGGGTCTAAATTGA

Antitoxin

Protein

• Length: 278 a.a.
• Molecular weight: 30877.26 Da
• Isoelectric Point: 5.7725

>AT209924 WP_003112902.1 NZ_CP079757:c3921065-3920232 [Pseudomonas aeruginosa]
MDKSDDSQDKYIVPGLERGLLLLCEFSRKDRTLTAPELARRLKLPRSTIFRLLTTLEAMGFVTRNGNEYRLGMAVLRLGF
EYLASLELTELGQPLLNRLCDEIRYPCNLVVRDGRSIVYVAKVSPSTPLSSSVNVGTRLPAHATVLGRILLQDLSLGELR
ELYPEEQLEQFSPNTPRSVLELFDMVQGDRQRGFVQGEGFFEASISTVAAPVRDHSGRVIAAMGATIAAGHIDPERIEGL
VSRVRSSADELSYLLDYRADGQDNVTPIFRSRPHETV

Nucleotide

• Length: 834 bp

>AT209924 NZ_CP104340:c1590479-1590075 [Acinetobacter baumannii]
ATGTTATATCCAATTGCAATTGAACGAGGATCAGATACTGAGGCATTTGGTGTCACTGTTCCTGATATTCCAGGTTGTTT
TAGTGCTGGTGACACACTTGAAGAAGCTATTGAGAATGTTAAAGAAGCTATTTCAGGCCATTTAGAAATATTGGCTGAAG
ATGGTGAGGAAATCCCATTAGCTTCCGAACTAGTTAAATTTGTCGATGATCCTGAATATAAAGGAATGATCTGGGCGGTT
ACCGAAGTTGATGTTAGTCGTTATCTGGGTAAACCAGAAAAAATCAATGTTACTTTACCAAGCCGTTTGATTCGTAAAAT
TGATGAGAATGTAGGTAAAGGTAAGAGATATACTACTCGATCGGCTTTCTTGGCTGCTGGTGCTGAAAAACTTTTACATG
CATAG

Structures

Toxin

• AlphaFold DB: Q9HYA0

Antitoxin

• AlphaFold DB: Q9HYA1