Detailed information of TA system TA209902

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1853906..1854496
• Replicon: chromosome
• Accession: NZ_CP079752
• Organism: Raoultella ornithinolytica strain NY1

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: KXJ76_RS08705
• Protein ID: WP_123629565.1
• Coordinates: 1854164..1854496 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: KXJ76_RS08700
• Protein ID: WP_123629566.1
• Coordinates: 1853906..1854163 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KXJ76_RS08675 (KXJ76_08675)	1849311..1850507	-	1197	WP_219173539.1	MFS transporter	-
KXJ76_RS08680 (KXJ76_08680)	1850504..1850899	-	396	WP_032686835.1	heme-binding protein	-
KXJ76_RS08685 (KXJ76_08685)	1850997..1851701	-	705	WP_065906778.1	IclR family transcriptional regulator	-
KXJ76_RS08690 (KXJ76_08690)	1851992..1852891	+	900	WP_162660979.1	alpha/beta hydrolase	-
KXJ76_RS08695 (KXJ76_08695)	1853427..1853558	+	132	Protein_1701	helix-turn-helix domain-containing protein	-
KXJ76_RS08700 (KXJ76_08700)	1853906..1854163	+	258	WP_123629566.1	antitoxin	Antitoxin
KXJ76_RS08705 (KXJ76_08705)	1854164..1854496	+	333	WP_123629565.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KXJ76_RS08710 (KXJ76_08710)	1854954..1855817	+	864	WP_004864372.1	DUF6387 family protein	-
KXJ76_RS08715 (KXJ76_08715)	1856005..1856223	+	219	WP_032737122.1	AlpA family transcriptional regulator	-
KXJ76_RS08720 (KXJ76_08720)	1856363..1856680	+	318	WP_000030115.1	hypothetical protein	-
KXJ76_RS08725 (KXJ76_08725)	1856746..1856982	+	237	WP_049100099.1	hypothetical protein	-
KXJ76_RS08730 (KXJ76_08730)	1857053..1857349	+	297	WP_004864365.1	hypothetical protein	-
KXJ76_RS08735 (KXJ76_08735)	1857412..1857654	+	243	WP_004864362.1	hypothetical protein	-
KXJ76_RS08740 (KXJ76_08740)	1857911..1858549	-	639	WP_004864361.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11783.65 Da
• Isoelectric Point: 9.4051

>T209902 WP_123629565.1 NZ_CP079752:1854164-1854496 [Raoultella ornithinolytica]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSTASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEGAGTKTIGVIRCDQPRTI
DMAARNGMRLERIPDAVVNEVLARLDAILS

Nucleotide

• Length: 333 bp

>T209902 NZ_CP104330:c3590133-3589735 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9469.00 Da
• Isoelectric Point: 4.6258

>AT209902 WP_123629566.1 NZ_CP079752:1853906-1854163 [Raoultella ornithinolytica]
MYKTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT209902 NZ_CP104330:c3590429-3590136 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA