Detailed information of TA system TA209857

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3434775..3435393
• Replicon: chromosome
• Accession: NZ_CP079736
• Organism: Escherichia coli strain GB2005-delet-a-f

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: KYX13_RS16750
• Protein ID: WP_001290581.1
• Coordinates: 3435175..3435393 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: S1PTH5
• Locus tag: KYX13_RS16745
• Protein ID: WP_000344800.1
• Coordinates: 3434775..3435149 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KYX13_RS16735 (3429864)	3429864..3431057	+	1194	WP_001295324.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
KYX13_RS16740 (3431080)	3431080..3434229	+	3150	WP_001132469.1	efflux RND transporter permease AcrB	-
KYX13_RS16745 (3434775)	3434775..3435149	+	375	WP_000344800.1	Hha toxicity modulator TomB	Antitoxin
KYX13_RS16750 (3435175)	3435175..3435393	+	219	WP_001290581.1	HHA domain-containing protein	Toxin
KYX13_RS16755 (3435565)	3435565..3436116	+	552	WP_000102564.1	maltose O-acetyltransferase	-
KYX13_RS16760 (3436232)	3436232..3436702	+	471	WP_000136192.1	YlaC family protein	-
KYX13_RS16765 (3436866)	3436866..3438416	+	1551	WP_001310610.1	cyclic-guanylate-specific phosphodiesterase PdeB	-
KYX13_RS16770 (3438458)	3438458..3438811	-	354	WP_000878140.1	DUF1428 domain-containing protein	-
KYX13_RS16780 (3439190)	3439190..3439501	+	312	WP_000409911.1	MGMT family protein	-
KYX13_RS16785 (3439532)	3439532..3440104	-	573	WP_000779842.1	YbaY family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8678.06 Da
• Isoelectric Point: 8.9008

>T209857 WP_001290581.1 NZ_CP079736:3435175-3435393 [Escherichia coli]
MSEKFLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T209857 NZ_CP104328:974980-975315 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14557.39 Da
• Isoelectric Point: 4.7395

>AT209857 WP_000344800.1 NZ_CP079736:3434775-3435149 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Nucleotide

• Length: 375 bp

>AT209857 NZ_CP104328:974732-974980 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9QBQ5