Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3633541..3634082 Replicon chromosome
Accession NZ_CP079735
Organism Escherichia coli strain GB2005

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag KYX12_RS17775 Protein ID WP_000615983.1
Coordinates 3633804..3634082 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag KYX12_RS17770 Protein ID WP_000729703.1
Coordinates 3633541..3633801 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYX12_RS17750 (3629216) 3629216..3630001 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
KYX12_RS17755 (3629973) 3629973..3631685 + 1713 Protein_3484 flagellar biosynthesis protein FlhA -
KYX12_RS17760 (3631909) 3631909..3632406 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
KYX12_RS17765 (3632582) 3632582..3633331 - 750 WP_000056849.1 C40 family peptidase -
KYX12_RS17770 (3633541) 3633541..3633801 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
KYX12_RS17775 (3633804) 3633804..3634082 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
KYX12_RS17780 (3634238) 3634238..3634978 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
KYX12_RS17785 (3634949) 3634949..3635716 - 768 WP_012305459.1 class II glutamine amidotransferase -
KYX12_RS17790 (3635922) 3635922..3636500 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T209833 WP_000615983.1 NZ_CP079735:3633804-3634082 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T209833 NZ_CP104326:1962364-1962467 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT209833 WP_000729703.1 NZ_CP079735:3633541-3633801 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT209833 NZ_CP104326:c1962505-1962234 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References