Detailed information of TA system TA209807

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 42826..43500
• Replicon: plasmid pKP3295-2
• Accession: NZ_CP079727
• Organism: Klebsiella pneumoniae strain KP3295

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5Q9LMS5
• Locus tag: KXI30_RS26730
• Protein ID: WP_032720638.1
• Coordinates: 42826..43149 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KXI30_RS26735
• Protein ID: WP_032720637.1
• Coordinates: 43198..43500 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KXI30_RS26710 (KXI30_26700)	37848..39251	-	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
KXI30_RS26715 (KXI30_26705)	39363..40895	+	1533	WP_023288255.1	IS3-like element ISKpn38 family transposase	-
KXI30_RS26720 (KXI30_26710)	41167..41769	+	603	Protein_44	RNA-guided endonuclease TnpB family protein	-
KXI30_RS28370	41838..42265	-	428	Protein_45	DNA-binding protein	-
KXI30_RS26725 (KXI30_26715)	42243..42648	+	406	Protein_46	DUF4113 domain-containing protein	-
KXI30_RS26730 (KXI30_26720)	42826..43149	+	324	WP_032720638.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KXI30_RS26735 (KXI30_26725)	43198..43500	+	303	WP_032720637.1	NadS family protein	Antitoxin
KXI30_RS26740 (KXI30_26730)	43543..43710	-	168	WP_153591440.1	hypothetical protein	-
KXI30_RS26745 (KXI30_26735)	43898..44104	-	207	WP_153591439.1	hypothetical protein	-
KXI30_RS26750 (KXI30_26740)	44101..44484	-	384	WP_032720636.1	hypothetical protein	-
KXI30_RS26755 (KXI30_26745)	44589..45161	-	573	WP_032720635.1	GNAT family N-acetyltransferase	-
KXI30_RS26760 (KXI30_26750)	45163..45975	-	813	WP_223203129.1	TSUP family transporter	-
KXI30_RS26765 (KXI30_26755)	45987..46907	-	921	WP_050548604.1	TauD/TfdA family dioxygenase	-
KXI30_RS26770 (KXI30_26760)	47210..47704	-	495	WP_044266755.1	hypothetical protein	-
KXI30_RS26775 (KXI30_26765)	47735..48307	-	573	WP_044266753.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	qnrB1 / dfrA14 / blaCTX-M-15 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2	-	1..133750	133750

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (45-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-83)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12372.29 Da
• Isoelectric Point: 9.6240

>T209807 WP_032720638.1 NZ_CP079727:42826-43149 [Klebsiella pneumoniae]
MNYLEFVETSAFSGLRKELMDDDEFRELQTYLLEAHDRGDTISHTGGCRKIRWGRPGMGKRGGVRVIYYVRLASGRLYLL
LIYPKNAKDELSEKEKAVMKALTQQLK

Nucleotide

• Length: 324 bp

>T209807 NZ_CP104312:4123555-4123773 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11211.12 Da
• Isoelectric Point: 7.3611

>AT209807 WP_032720637.1 NZ_CP079727:43198-43500 [Klebsiella pneumoniae]
MEKKLFDALISSMEEMVAIENGQIQPAAEQVHRHTLPDVKAIRKHAGMKQAEFAEVVGASVDLVRSWEQQRRVPSGIALK
MLCLIEREPALINTLRGIHV

Nucleotide

• Length: 303 bp

>AT209807 NZ_CP104312:4123154-4123528 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A5Q9LMS5