Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4680099..4680909 Replicon chromosome
Accession NZ_CP079683
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC30_AI1547MPCH

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag KW563_RS22900 Protein ID WP_004178461.1
Coordinates 4680099..4680632 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KW563_RS22905 Protein ID WP_002887278.1
Coordinates 4680643..4680909 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW563_RS22895 (4678930) 4678930..4680051 + 1122 WP_004214138.1 cupin domain-containing protein -
KW563_RS22900 (4680099) 4680099..4680632 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
KW563_RS22905 (4680643) 4680643..4680909 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KW563_RS22910 (4681012) 4681012..4682445 - 1434 WP_064162551.1 Cu(+)/Ag(+) sensor histidine kinase -
KW563_RS22915 (4682435) 4682435..4683118 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
KW563_RS22920 (4683290) 4683290..4684675 + 1386 WP_064162550.1 efflux transporter outer membrane subunit -
KW563_RS22925 (4684693) 4684693..4685037 + 345 WP_064162549.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T209710 WP_004178461.1 NZ_CP079683:c4680632-4680099 [Klebsiella pneumoniae subsp. pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T209710 NZ_CP104284:c1169088-1168777 [Enterobacter hormaechei subsp. steigerwaltii]
ATGCACATAGTCTCAAGAGCGCCTTTCGACACAGCGGCCAGACATTATCCCAATCAAGCACAAGCCCTGGACGATCTTTA
CCGGGTACTAAAAAAGGAACACTACCCCTCACCAGACGAGATGCGAAAGCGCTTTCCTAGCCTGGACCGGATGAAATACC
GGGAAAAATGGTGGGTCATTGATGTGGGTGGCGGGCATCTAAGAGTGATGTTTTTTGCTGATTTTGAGCGTGGAAAGATC
TTCATCAAACACATCAGCACTCACGCAGACTATGACAAGCTCACGGACTACTACCGGAGAACGAAAGAATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT209710 WP_002887278.1 NZ_CP079683:c4680909-4680643 [Klebsiella pneumoniae subsp. pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT209710 NZ_CP104284:c1168780-1168361 [Enterobacter hormaechei subsp. steigerwaltii]
ATGAATTATGCAGCAGCCATCAAAGCGGCAAACGCTCTGACAAACGAGCTCCCTTTCCTGGGTTCAAGCCCGTCGCGGCA
AGACTACGAGGATGCGCTGGCGCTGGTGGAGTATCTTATCGAGCACGACCCCGATAACCCGCTGGTAGATATGCTCACTG
CCAAAATCGATAAGTATGAAAACGCGTCCCCGGAGTTTGCAGAATTTAACGCGCGTATTGCCAGCATTCCGTCAGGCGTT
GCGTTACTGCGAACGCTAATGGATCAGTACCAGCTCACTCAGAGTGATTTCGAGAACGAGATTGGTAAGAAGTCTCTGGT
AAGTCGGATCCTTAACGGACAGCGCACGTTAACGCTAGATCATATGCGAGCGCTGGCGAAGCGTTTCGGTTTGCCGGTGA
GCGCATTTGTGGGGAACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References