Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 4183974..4184490 Replicon chromosome
Accession NZ_CP079599
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC10_CMC309MC

Toxin (Protein)


Gene name relE Uniprot ID R4YAY3
Locus tag KWY78_RS20295 Protein ID WP_004178374.1
Coordinates 4184206..4184490 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID R4Y888
Locus tag KWY78_RS20290 Protein ID WP_002886901.1
Coordinates 4183974..4184216 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWY78_RS20275 (4180002) 4180002..4180742 + 741 WP_002886899.1 KDGP aldolase family protein -
KWY78_RS20280 (4180809) 4180809..4181963 + 1155 WP_004178372.1 lactonase family protein -
KWY78_RS20285 (4181986) 4181986..4183896 + 1911 WP_004178373.1 PRD domain-containing protein -
KWY78_RS20290 (4183974) 4183974..4184216 + 243 WP_002886901.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
KWY78_RS20295 (4184206) 4184206..4184490 + 285 WP_004178374.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KWY78_RS20300 (4184494) 4184494..4184958 - 465 WP_004178375.1 anaerobic ribonucleoside-triphosphate reductase-activating protein -
KWY78_RS20305 (4185199) 4185199..4187337 - 2139 WP_004186701.1 anaerobic ribonucleoside-triphosphate reductase -
KWY78_RS20310 (4187694) 4187694..4188437 - 744 WP_002886905.1 MurR/RpiR family transcriptional regulator -
KWY78_RS20315 (4188440) 4188440..4188613 - 174 WP_032408826.1 hypothetical protein -
KWY78_RS20320 (4188698) 4188698..4189006 + 309 WP_004178377.1 PTS sugar transporter subunit IIB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-80)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 11155.97 Da        Isoelectric Point: 10.3787

>T209485 WP_004178374.1 NZ_CP079599:4184206-4184490 [Klebsiella pneumoniae subsp. pneumoniae]
MTYELEFDPRAWREWQKPGETVKKQFKNKLQQIVQNPRIESTRLSDLPDCYKIKLKASGYRLVYQVRDSVVVVYVIAIGK
REKAAVYHQANKRL

Download         Length: 285 bp

>T209485 NZ_CP104114:c3951609-3951313 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9045.55 Da        Isoelectric Point: 6.3340

>AT209485 WP_002886901.1 NZ_CP079599:4183974-4184216 [Klebsiella pneumoniae subsp. pneumoniae]
MATLNVRLDDKLKKEAYAVLEKLDMTPTEAVRLLFQYIAENGRMPVKSMTISDGEAALLRTVRERLENPRPGVKVSLDDL

Download         Length: 243 bp

>AT209485 NZ_CP104114:c3951311-3950916 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A6THG1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GLP0

References