Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3714214..3714839 Replicon chromosome
Accession NZ_CP079599
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC10_CMC309MC

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KWY78_RS18050 Protein ID WP_002882817.1
Coordinates 3714456..3714839 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KWY78_RS18045 Protein ID WP_004150355.1
Coordinates 3714214..3714456 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWY78_RS18020 (3710205) 3710205..3710804 + 600 WP_004151865.1 glucose-1-phosphatase -
KWY78_RS18025 (3710798) 3710798..3711658 + 861 WP_004146232.1 virulence factor BrkB family protein -
KWY78_RS18030 (3711655) 3711655..3712092 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KWY78_RS18035 (3712137) 3712137..3713078 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KWY78_RS18040 (3713092) 3713092..3714009 - 918 WP_004181612.1 alpha/beta hydrolase -
KWY78_RS18045 (3714214) 3714214..3714456 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KWY78_RS18050 (3714456) 3714456..3714839 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KWY78_RS18055 (3715013) 3715013..3715942 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KWY78_RS18060 (3715939) 3715939..3716574 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KWY78_RS18065 (3716571) 3716571..3717473 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T209483 WP_002882817.1 NZ_CP079599:3714456-3714839 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T209483 NZ_CP104114:c3649451-3649116 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT209483 WP_004150355.1 NZ_CP079599:3714214-3714456 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT209483 NZ_CP104114:c3649699-3649451 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References