Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4697880..4698505 Replicon chromosome
Accession NZ_CP079120
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC03_AI1235PC

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KWV87_RS22915 Protein ID WP_002882817.1
Coordinates 4698122..4698505 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KWV87_RS22910 Protein ID WP_004150355.1
Coordinates 4697880..4698122 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWV87_RS22885 (4693871) 4693871..4694470 + 600 WP_004151865.1 glucose-1-phosphatase -
KWV87_RS22890 (4694464) 4694464..4695324 + 861 WP_004146232.1 virulence factor BrkB family protein -
KWV87_RS22895 (4695321) 4695321..4695758 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KWV87_RS22900 (4695803) 4695803..4696744 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KWV87_RS22905 (4696758) 4696758..4697675 - 918 WP_004181612.1 alpha/beta hydrolase -
KWV87_RS22910 (4697880) 4697880..4698122 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KWV87_RS22915 (4698122) 4698122..4698505 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KWV87_RS22920 (4698679) 4698679..4699608 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KWV87_RS22925 (4699605) 4699605..4700240 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KWV87_RS22930 (4700237) 4700237..4701139 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T209264 WP_002882817.1 NZ_CP079120:4698122-4698505 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T209264 NZ_CP103995:c1439113-1438367 [Bacillus subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTATATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATCCGGAAAACTTGGTATTTGCTGTTTGTTCTAGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATACGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACTGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT209264 WP_004150355.1 NZ_CP079120:4697880-4698122 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT209264 NZ_CP103995:c1438367-1438197 [Bacillus subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References