Detailed information of TA system TA209248

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 13599..14256
• Replicon: plasmid pS465_P1
• Accession: NZ_CP079116
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WRC01_S465PC

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: KWK18_RS27015
• Protein ID: WP_000270043.1
• Coordinates: 13906..14256 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KWK18_RS27010
• Protein ID: WP_000124640.1
• Coordinates: 13599..13901 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KWK18_RS26960 (KWK18_26960)	9233..9661	+	429	WP_000591076.1	hypothetical protein	-
KWK18_RS26965 (KWK18_26965)	9719..10078	+	360	WP_000422769.1	hypothetical protein	-
KWK18_RS26970 (KWK18_26970)	10078..10524	+	447	WP_000919343.1	hypothetical protein	-
KWK18_RS26975 (KWK18_26975)	10521..11039	+	519	WP_000210757.1	nitrite reductase	-
KWK18_RS26980 (KWK18_26980)	11039..11269	+	231	WP_000972665.1	hypothetical protein	-
KWK18_RS26985 (KWK18_26985)	11256..12113	+	858	WP_001167036.1	hypothetical protein	-
KWK18_RS28420	12139..12330	+	192	WP_001270409.1	hypothetical protein	-
KWK18_RS26995 (KWK18_26995)	12333..12860	+	528	WP_004201083.1	thermonuclease family protein	-
KWK18_RS27000 (KWK18_27000)	12918..13190	+	273	WP_001043046.1	HU family DNA-binding protein	-
KWK18_RS27005 (KWK18_27005)	13279..13572	+	294	WP_001239998.1	hypothetical protein	-
KWK18_RS27010 (KWK18_27010)	13599..13901	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
KWK18_RS27015 (KWK18_27015)	13906..14256	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KWK18_RS27020 (KWK18_27020)	14419..14967	+	549	WP_001061573.1	hypothetical protein	-
KWK18_RS27025 (KWK18_27025)	15308..15502	+	195	WP_000343597.1	hypothetical protein	-
KWK18_RS27030 (KWK18_27030)	15513..15884	+	372	WP_000516918.1	hypothetical protein	-
KWK18_RS27035 (KWK18_27035)	15877..16347	+	471	WP_001281821.1	hypothetical protein	-
KWK18_RS27040 (KWK18_27040)	16362..16697	-	336	WP_000683477.1	hypothetical protein	-
KWK18_RS27045 (KWK18_27045)	16794..17282	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
KWK18_RS27050 (KWK18_27050)	17285..17782	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-4 / dfrA12 / aadA2 / qacE / sul1 / armA / msr(E) / mph(E) / blaOXA-1 / aac(6')-Ib-cr / qnrB1 / tet(D) / blaTEM-1B	-	1..210997	210997

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T209248 WP_000270043.1 NZ_CP079116:c14256-13906 [Klebsiella pneumoniae subsp. pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T209248 NZ_CP103974:2888366-2888473 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT209248 WP_000124640.1 NZ_CP079116:c13901-13599 [Klebsiella pneumoniae subsp. pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT209248 NZ_CP103974:c2888319-2888253 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9