Detailed information of TA system TA209128

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2679549..2681137
• Replicon: chromosome
• Accession: NZ_CP078146
• Organism: Klebsiella variicola strain JNQH579

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: KW408_RS12885
• Protein ID: WP_023297605.1
• Coordinates: 2679815..2681137 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A1F2MCN9
• Locus tag: KW408_RS12880
• Protein ID: WP_008804920.1
• Coordinates: 2679549..2679815 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KW408_RS12855 (KW408_12855)	2674699..2675622	+	924	WP_211744035.1	LysR family transcriptional regulator	-
KW408_RS12860 (KW408_12860)	2675603..2676757	-	1155	WP_115656298.1	FAD-dependent urate hydroxylase HpxO	-
KW408_RS12865 (KW408_12865)	2677103..2678482	+	1380	WP_211744036.1	nucleobase:cation symporter-2 family protein	-
KW408_RS12870 (KW408_12870)	2678460..2678960	+	501	WP_023297604.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
KW408_RS12875 (KW408_12875)	2678957..2679283	+	327	WP_008804921.1	hydroxyisourate hydrolase	-
KW408_RS12880 (KW408_12880)	2679549..2679815	+	267	WP_008804920.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
KW408_RS12885 (KW408_12885)	2679815..2681137	+	1323	WP_023297605.1	type II toxin-antitoxin system HipA family toxin	Toxin
KW408_RS12890 (KW408_12890)	2681154..2682446	-	1293	WP_012968243.1	glycoside hydrolase family 10 protein	-
KW408_RS12895 (KW408_12895)	2682579..2683583	-	1005	WP_211744038.1	fimbrial protein	-
KW408_RS12900 (KW408_12900)	2683571..2686072	-	2502	WP_110210726.1	fimbria/pilus outer membrane usher protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49010.40 Da
• Isoelectric Point: 8.2822

>T209128 WP_023297605.1 NZ_CP078146:2679815-2681137 [Klebsiella variicola]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPREEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGGHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEFLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMAFLLGSSEALKDRADFMKFMVFQWLVGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPGEQMLAILQEFAGHVP
QAIESARRTLPADFSTHVWRAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T209128 NZ_CP103860:777500-777604 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTTATGGTTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTACTCAAGACCATAAAAAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.30 Da
• Isoelectric Point: 8.5912

>AT209128 WP_008804920.1 NZ_CP078146:2679549-2679815 [Klebsiella variicola]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKATETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT209128 NZ_CP103860:c777763-777599 [Staphylococcus aureus]
GTAGTAAGTAGAAGCAAAAGATGAAAATCATTAACTCTTGAAACACAAAAAGGGCAACACTCGGAAACATGTTACCCTAA
TGAGCCCGTTAAAAAGACGGTGACCTTATATTTTATTTAAAAATAGCCTTCAAAAATGCCGGTCAAAGCGAATAGAAGGT
TATTT

Structures

Antitoxin

• AlphaFold DB: A0A1F2MCN9