Detailed information of TA system TA209060

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 106116..106369
• Replicon: plasmid pKP169-P2
• Accession: NZ_CP078123
• Organism: Klebsiella pneumoniae strain KPC-2

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: KU889_RS28145
• Protein ID: WP_001312851.1
• Coordinates: 106116..106265 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	106310..106369 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU889_RS28110 (101475)	101475..101890	-	416	Protein_136	IS1-like element IS1B family transposase	-
KU889_RS28115 (102139)	102139..102540	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
KU889_RS28120 (102473)	102473..102730	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
KU889_RS28125 (102823)	102823..103476	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
KU889_RS28130 (104415)	104415..105272	-	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
KU889_RS31120 (105265)	105265..105339	-	75	WP_001375168.1	RepA leader peptide Tap	-
KU889_RS28140 (105584)	105584..105832	-	249	WP_000083837.1	replication regulatory protein RepA	-
KU889_RS28145 (106116)	106116..106265	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (106310)	106310..106369	+	60	NuclAT_1	-	Antitoxin
- (106310)	106310..106369	+	60	NuclAT_1	-	Antitoxin
- (106310)	106310..106369	+	60	NuclAT_1	-	Antitoxin
- (106310)	106310..106369	+	60	NuclAT_1	-	Antitoxin
KU889_RS28150 (106570)	106570..106902	-	333	WP_152916585.1	hypothetical protein	-
KU889_RS28155 (106964)	106964..107563	-	600	WP_032083981.1	PIN domain-containing protein	-
KU889_RS28160 (107949)	107949..108149	-	201	WP_015059022.1	hypothetical protein	-
KU889_RS28165 (108281)	108281..108841	-	561	WP_000139328.1	fertility inhibition protein FinO	-
KU889_RS28170 (108896)	108896..109642	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
KU889_RS28175 (109662)	109662..109862	-	201	WP_072354025.1	hypothetical protein	-
KU889_RS28180 (109887)	109887..110591	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
KU889_RS28185 (110643)	110643..110987	+	345	Protein_151	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-2 / blaSHV-12 / rmtB / blaTEM-1B / fosA3	-	1..157394	157394

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T209060 WP_001312851.1 NZ_CP078123:c106265-106116 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T209060 NZ_CP103819:c1271051-1270680 [Actinobacillus equuli subsp. haemolyticus]
ATGTATGAAATAGTCTTTTATCGAGACCGACGTGGGCGAGAACCCGTCAAGGAATTCCTGCAGGAATTTATTAATGAACA
ACAAGACGAAAATCGAGAGCGTTTGCACAAAATTAGTCATCATTTGACAATTTTACATTTGCACGGCACTCGAGCAGGCG
AAAGCTATATTAAGCATTTAGAAGATCGTATTTGGCAGTTACGTCCAATTAGCGATTGTTTGCTCTTCGCCGGTATTGTG
CGAGGTCAGTTTGTATTGTTGCATCATTTCGCCAAAAGCAGTAGTCGGCTTCCTAAGCGTGAGCTTGAGCGAGCAAAAAG
TCGTTTGGCAGATCTACAGGAGAGGATAAAAGATGAGCCCCATTGGTTCTAG

Antitoxin

Nucleotide

• Length: 60 bp

>AT209060 NZ_CP078123:106310-106369 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin