Detailed information of TA system TA208991

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1604585..1605255
• Replicon: chromosome
• Accession: NZ_CP078110
• Organism: Cronobacter sakazakii strain MOD1-H322

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: BTK77_RS07580
• Protein ID: WP_080320917.1
• Coordinates: 1604585..1604881 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BTK77_RS07585
• Protein ID: WP_080320916.1
• Coordinates: 1604914..1605255 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTK77_RS07560 (BTK77_007560)	1600201..1601028	-	828	WP_004387342.1	ammonia-dependent NAD(+) synthetase	-
BTK77_RS07565 (BTK77_007565)	1601271..1601612	+	342	WP_004387341.1	osmotically-inducible lipoprotein OsmE	-
BTK77_RS07570 (BTK77_007570)	1601724..1603979	-	2256	WP_080320918.1	catalase HPII	-
BTK77_RS07575 (BTK77_007575)	1604191..1604421	+	231	WP_054624666.1	cell division activator CedA	-
BTK77_RS07580 (BTK77_007580)	1604585..1604881	+	297	WP_080320917.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BTK77_RS07585 (BTK77_007585)	1604914..1605255	+	342	WP_080320916.1	HigA family addiction module antitoxin	Antitoxin
BTK77_RS07590 (BTK77_007590)	1605367..1606758	-	1392	WP_054624667.1	L-cystine transporter	-
BTK77_RS07595 (BTK77_007595)	1606893..1607483	-	591	WP_004386566.1	metal-dependent hydrolase	-
BTK77_RS07600 (BTK77_007600)	1607597..1608352	-	756	WP_080320915.1	2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase KduD	-
BTK77_RS07605 (BTK77_007605)	1608539..1609207	-	669	WP_080320914.1	hexitol phosphatase HxpB	-
BTK77_RS07610 (BTK77_007610)	1609366..1609902	+	537	WP_080320913.1	YniB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (7-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11429.94 Da
• Isoelectric Point: 9.6980

>T208991 WP_080320917.1 NZ_CP078110:1604585-1604881 [Cronobacter sakazakii]
MQKHIGSFRDAWLAAFFVYSTPHRNIPAEIHTTLARKLDIINAATSYRDLRSPPGNRFEALSGKLQGYSSIRVNHHYRLI
FRWVEGKAEDLYLDPHDY

Nucleotide

• Length: 297 bp

>T208991 NZ_CP103771:c4498321-4497836 [Salmonella enterica]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTCGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATCCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12704.65 Da
• Isoelectric Point: 8.0136

>AT208991 WP_080320916.1 NZ_CP078110:1604914-1605255 [Cronobacter sakazakii]
MKQATRKPTTPGEILVNEYLEPLGLKINEVAEMLCVHRNSVSALVNNNRKLTTDMAFRLAKAFGTSVDFWLNLQAAVDLW
EVENDMRIQEAFSRIVPAAEFIARRDAQKKAGA

Nucleotide

• Length: 342 bp

>AT208991 NZ_CP103771:c4498596-4498309 [Salmonella enterica]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCGGGCTGCCGAGAATGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA