Detailed information of TA system TA208957

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-Phd
• Location: 4656149..4656832
• Replicon: chromosome
• Accession: NZ_CP078066
• Organism: Bradyrhizobium arachidis strain Hy4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KUL72_RS22465
• Protein ID: WP_258762024.1
• Coordinates: 4656149..4656586 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: KUL72_RS22470
• Protein ID: WP_258762025.1
• Coordinates: 4656590..4656832 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUL72_RS22440 (KUL72_22260)	4651715..4652164	+	450	WP_258762020.1	Lrp/AsnC family transcriptional regulator	-
KUL72_RS22445 (KUL72_22265)	4652175..4652672	+	498	WP_258762021.1	peptide deformylase	-
KUL72_RS22450 (KUL72_22270)	4652690..4652974	-	285	WP_258762022.1	hypothetical protein	-
KUL72_RS22455 (KUL72_22275)	4652982..4654331	+	1350	WP_258762023.1	IS701 family transposase	-
KUL72_RS22460 (KUL72_22280)	4654818..4656058	-	1241	Protein_4447	beta-lactamase family protein	-
KUL72_RS22465 (KUL72_22285)	4656149..4656586	-	438	WP_258762024.1	type II toxin-antitoxin system VapC family toxin	Toxin
KUL72_RS22470 (KUL72_22290)	4656590..4656832	-	243	WP_258762025.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KUL72_RS22475 (KUL72_22295)	4657052..4657351	+	300	WP_258762026.1	hypothetical protein	-
KUL72_RS22480 (KUL72_22300)	4657348..4657773	+	426	WP_258762027.1	PIN domain-containing protein	-
KUL72_RS22485 (KUL72_22305)	4657797..4658660	+	864	WP_258762028.1	hypothetical protein	-
KUL72_RS22490 (KUL72_22310)	4658678..4660276	-	1599	WP_258762029.1	glutamine-hydrolyzing GMP synthase	-
KUL72_RS22495 (KUL72_22315)	4660425..4660796	+	372	WP_258762030.1	nuclear transport factor 2 family protein	-
KUL72_RS22500 (KUL72_22320)	4661034..4661366	+	333	WP_258762031.1	DHCW motif cupin fold protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4653018..4654331	1313

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-63)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15758.06 Da
• Isoelectric Point: 6.6455

>T208957 WP_258762024.1 NZ_CP078066:c4656586-4656149 [Bradyrhizobium arachidis]
VYLVDTNVISAVSPNRLAPAALVEWMDAHSASLFLSAVTIAEVEDGIAKLRREKATRRSKDLAQWLDVVLHLYGDRILAF
DTAVARLAGELSDRARGQGHAPGFADIIIAATARQHALTILSRNVRHFEPLGVPVLDPFSELPPS

Nucleotide

• Length: 438 bp

>T208957 NZ_CP103762:3056439-3056933 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8972.40 Da
• Isoelectric Point: 11.2765

>AT208957 WP_258762025.1 NZ_CP078066:c4656832-4656590 [Bradyrhizobium arachidis]
MREIQLRDAKANLSAVVDEAIDGKPAVITRHGKKQAVVLSYEEWRRLSQVPSFGRLLMAAPARAGDLPARNRSKLRKTDF

Nucleotide

• Length: 243 bp

>AT208957 NZ_CP103762:3056149-3056442 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAACCAGGCGCGAATCTTTAAATTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA