Detailed information of TA system TA208954

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 40590..41175
• Replicon: plasmid pAJ_068-2
• Accession: NZ_CP078043
• Organism: Acinetobacter junii strain AJ_068

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: EGT68_RS15415
• Protein ID: WP_000897307.1
• Coordinates: 40590..40910 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EGT68_RS15420
• Protein ID: WP_000369781.1
• Coordinates: 40903..41175 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGT68_RS15395 (EGT68_015395)	36229..36935	+	707	Protein_38	IS1 family transposase	-
EGT68_RS15400 (EGT68_015400)	37068..38393	-	1326	WP_020846983.1	ISNCY family transposase	-
EGT68_RS15405 (EGT68_015405)	38544..38963	-	420	WP_001180106.1	SMI1/KNR4 family protein	-
EGT68_RS15410 (EGT68_015410)	39141..40466	+	1326	WP_218999232.1	ISNCY family transposase	-
EGT68_RS15415 (EGT68_015415)	40590..40910	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EGT68_RS15420 (EGT68_015420)	40903..41175	+	273	WP_000369781.1	NadS family protein	Antitoxin
EGT68_RS15425 (EGT68_015425)	41271..42605	-	1335	WP_125272815.1	ISNCY family transposase	-
EGT68_RS15430 (EGT68_015430)	43154..44629	+	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
EGT68_RS15435 (EGT68_015435)	44685..45569	+	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaOXA-58 / msr(E) / mph(E) / aph(6)-Id / aac(3)-IId / aph(3')-VIa / sul1 / qacE / ARR-3 / aph(3')-VI / blaNDM-1 / sul2 / aph(3'')-Ib / floR	-	1..264416	264416
	-	inside	IScluster/Tn	blaOXA-58 / msr(E) / mph(E) / aph(6)-Id / aac(3)-IId	-	34769..57366	22597

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T208954 WP_000897307.1 NZ_CP078043:40590-40910 [Acinetobacter junii]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T208954 NZ_CP103762:2513929-2514624 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAATGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAACCGTTATGCCAGACCGTTATCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
TGAAACTATTCAGGCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTATTGGGTGGCG
CAGGAATACACATCAGCGATCTCAAACTGGCAATGGGGGTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATT
TATCCGCGACATTTTCTGGCGACAGCAAAGGTGCTGAAATTTCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGC
CAGAATGATTCCGGCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTG
AAACCAATGTGTTGAGGTTGCACGGCCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT208954 WP_000369781.1 NZ_CP078043:40903-41175 [Acinetobacter junii]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT208954 NZ_CP103762:2513663-2513929 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTAGCGAATGCAATGAAACTGGTTCGTCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATCGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TGACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3