Detailed information of TA system TA208946

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ygfYX/Cpta(toxin)
• Location: 1273371..1274040
• Replicon: chromosome
• Accession: NZ_CP078038
• Organism: Shewanella putrefaciens strain SA70

Toxin (Protein)

• Gene name: ygfX
• Uniprot ID: -
• Locus tag: B7D78_RS23630
• Protein ID: WP_176370316.1
• Coordinates: 1273600..1274040 (+)
• Length: 147 a.a.

Antitoxin (Protein)

• Gene name: ygfY
• Uniprot ID: -
• Locus tag: B7D78_RS05640
• Protein ID: WP_084294861.1
• Coordinates: 1273371..1273619 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7D78_RS05615 (KVP08_005615)	1269008..1269814	+	807	WP_011623612.1	prolipoprotein diacylglyceryl transferase	-
B7D78_RS05620 (KVP08_005620)	1269814..1270608	+	795	WP_086903299.1	thymidylate synthase	-
B7D78_RS05625 (KVP08_005625)	1270726..1271895	+	1170	WP_086903300.1	Na+/H+ antiporter NhaA	-
B7D78_RS05630 (KVP08_005630)	1271898..1272284	+	387	WP_086903301.1	hypothetical protein	-
B7D78_RS05635 (KVP08_005635)	1272380..1273288	+	909	WP_086903346.1	transcriptional activator NhaR	-
B7D78_RS05640 (KVP08_005640)	1273371..1273619	+	249	WP_084294861.1	succinate dehydrogenase assembly factor 2	Antitoxin
B7D78_RS23630	1273600..1274040	+	441	WP_176370316.1	hypothetical protein	Toxin
B7D78_RS05645 (KVP08_005645)	1274145..1275758	-	1614	WP_086903302.1	L-aspartate oxidase	-
B7D78_RS05650 (KVP08_005650)	1275948..1276526	+	579	WP_011623604.1	RNA polymerase sigma factor RpoE	-
B7D78_RS05655 (KVP08_005655)	1276553..1277176	+	624	WP_086903303.1	RseA family anti-sigma factor	-
B7D78_RS05660 (KVP08_005660)	1277189..1278121	+	933	WP_086903304.1	MucB/RseB C-terminal domain-containing protein	-
B7D78_RS05665 (KVP08_005665)	1278133..1278606	+	474	WP_086903305.1	SoxR reducing system RseC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (5-134)

Antitoxin

• Flavinator of succinate dehydrogenase (7-77)

Sequences

Toxin

Protein

• Length: 147 a.a.
• Molecular weight: 17129.11 Da
• Isoelectric Point: 9.2466

>T208946 WP_176370316.1 NZ_CP078038:1273600-1274040 [Shewanella putrefaciens]
VAERRHSFSVKASRDQRLSLVVFVCVCSTSLLIWPETDVVFWRVLKLIFAVLILGFLGYQLWRLRTWHCRFELNGKGEGW
LSTGEAFHVLPRTWVTPFVCLVYYQSADKLYLLPLWADMFSDTDYRHLCRLLLKVKTQAASQRESL

Nucleotide

• Length: 441 bp

>T208946 NZ_CP103762:c824536-824159 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9559.09 Da
• Isoelectric Point: 4.3313

>AT208946 WP_084294861.1 NZ_CP078038:1273371-1273619 [Shewanella putrefaciens]
MELMNIARVRWACRRGMLELDVLFQPFVENVYQDLSDDDKALFIRLLECEDPELFAWFMGHEACPDAELARMVVQVRGRA
AP

Nucleotide

• Length: 249 bp

>AT208946 NZ_CP103762:c824994-824626 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGACATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA