Detailed information of TA system TA208943

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 39867..40293
• Replicon: plasmid pKP_160-4
• Accession: NZ_CP078036
• Organism: Klebsiella pneumoniae strain KP_160

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: EGK18_RS28735
• Protein ID: WP_001372321.1
• Coordinates: 39867..39992 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	40069..40293 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGK18_RS28700 (34890)	34890..35117	-	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-
EGK18_RS28705 (35254)	35254..35925	-	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
EGK18_RS28710 (36119)	36119..36502	-	384	WP_001354030.1	conjugal transfer relaxosome DNA-binding protein TraM	-
EGK18_RS28715 (36837)	36837..37427	+	591	WP_000252683.1	transglycosylase SLT domain-containing protein	-
EGK18_RS28720 (37724)	37724..38545	-	822	WP_001234445.1	DUF932 domain-containing protein	-
EGK18_RS28725 (38656)	38656..38952	-	297	WP_001272251.1	hypothetical protein	-
EGK18_RS28730 (39252)	39252..39548	+	297	Protein_46	hypothetical protein	-
EGK18_RS28735 (39867)	39867..39992	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
EGK18_RS29240 (39934)	39934..40083	-	150	Protein_48	plasmid maintenance protein Mok	-
- (40069)	40069..40293	-	225	NuclAT_0	-	Antitoxin
- (40069)	40069..40293	-	225	NuclAT_0	-	Antitoxin
- (40069)	40069..40293	-	225	NuclAT_0	-	Antitoxin
- (40069)	40069..40293	-	225	NuclAT_0	-	Antitoxin
EGK18_RS28740 (40305)	40305..41024	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
EGK18_RS28745 (41021)	41021..41455	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
EGK18_RS28750 (41524)	41524..43547	-	2024	Protein_51	ParB/RepB/Spo0J family partition protein	-
EGK18_RS28755 (43608)	43608..43841	-	234	WP_000006003.1	DUF905 family protein	-
EGK18_RS28760 (43899)	43899..44426	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
EGK18_RS29245 (44728)	44728..45177	+	450	Protein_54	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T208943 WP_001372321.1 NZ_CP078036:c39992-39867 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T208943 NZ_CP103762:217147-217254 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CATGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT208943 NZ_CP078036:c40293-40069 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin