Detailed information of TA system TA208834

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5499865..5500460
• Replicon: chromosome
• Accession: NZ_CP077997
• Organism: Pseudomonas aeruginosa strain SRRSH1521

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: KUU75_RS25675
• Protein ID: WP_003113526.1
• Coordinates: 5500182..5500460 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KUU75_RS25670
• Protein ID: WP_003113527.1
• Coordinates: 5499865..5500170 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUU75_RS25650 (KUU75_25645)	5495941..5496198	+	258	WP_010793759.1	ATP-binding protein	-
KUU75_RS25655 (KUU75_25650)	5496574..5497437	-	864	WP_010793758.1	integrase domain-containing protein	-
KUU75_RS25660 (KUU75_25655)	5498039..5499181	-	1143	WP_010793757.1	STY4528 family pathogenicity island replication protein	-
KUU75_RS25670 (KUU75_25665)	5499865..5500170	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
KUU75_RS25675 (KUU75_25670)	5500182..5500460	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KUU75_RS31250	5500513..5500641	-	129	Protein_5093	integrase	-
KUU75_RS25680 (KUU75_25675)	5500789..5503017	+	2229	WP_003099265.1	TonB-dependent receptor	-
KUU75_RS25685 (KUU75_25680)	5503087..5503734	-	648	WP_003095021.1	carbonate dehydratase	-
KUU75_RS25690 (KUU75_25685)	5503796..5505034	-	1239	WP_010793756.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T208834 WP_003113526.1 NZ_CP077997:c5500460-5500182 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T208834 NZ_CP103742:c818353-817976 [Escherichia coli O25b:H4-ST131]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCAGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT208834 WP_003113527.1 NZ_CP077997:c5500170-5499865 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT208834 NZ_CP103742:c818810-818442 [Escherichia coli O25b:H4-ST131]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCTCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCCTGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCGGATGCGTACCATCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACCGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: V6ALY3