Detailed information of TA system TA208833

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4562967..4564225
• Replicon: chromosome
• Accession: NZ_CP077997
• Organism: Pseudomonas aeruginosa strain SRRSH1521

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9I4U4
• Locus tag: KUU75_RS21120
• Protein ID: WP_003112528.1
• Coordinates: 4562967..4563722 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: KUU75_RS21125
• Protein ID: WP_003086329.1
• Coordinates: 4563857..4564225 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUU75_RS21110 (KUU75_21105)	4558140..4560683	+	2544	WP_003112526.1	acyl-homoserine-lactone acylase QuiP	-
KUU75_RS21115 (KUU75_21110)	4560838..4562199	-	1362	WP_010895536.1	DNA recombination protein RmuC	-
KUU75_RS21120 (KUU75_21115)	4562967..4563722	-	756	WP_003112528.1	RES family NAD+ phosphorylase	Toxin
KUU75_RS21125 (KUU75_21120)	4563857..4564225	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
KUU75_RS21130 (KUU75_21125)	4564298..4565584	-	1287	WP_003112529.1	FAD-binding oxidoreductase	-
KUU75_RS21135 (KUU75_21130)	4565640..4567229	-	1590	WP_003123191.1	aldehyde dehydrogenase family protein	-
KUU75_RS21140 (KUU75_21135)	4567308..4567784	+	477	WP_003112531.1	hypothetical protein	-
KUU75_RS21145 (KUU75_21140)	4567787..4568062	-	276	WP_010793902.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28278.42 Da
• Isoelectric Point: 7.7294

>T208833 WP_003112528.1 NZ_CP077997:c4563722-4562967 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T208833 NZ_CP103742:642568-642882 [Escherichia coli O25b:H4-ST131]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT208833 WP_003086329.1 NZ_CP077997:c4564225-4563857 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT208833 NZ_CP103742:642879-643295 [Escherichia coli O25b:H4-ST131]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: Q9I4U4

Antitoxin

• AlphaFold DB: V6AJU7