Detailed information of TA system TA208645

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TscAT/-
• Location: 959334..959863
• Replicon: chromosome
• Accession: NZ_CP077925
• Organism: Staphylococcus aureus strain 198

Toxin (Protein)

• Gene name: TscT
• Uniprot ID: K7ZRX6
• Locus tag: KU531_RS04645
• Protein ID: WP_001103939.1
• Coordinates: 959546..959863 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: TscA
• Uniprot ID: X5IY59
• Locus tag: KU531_RS04640
• Protein ID: WP_001058494.1
• Coordinates: 959334..959543 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU531_RS04600 (954755)	954755..954931	+	177	WP_168240022.1	hypothetical protein	-
KU531_RS04605 (954928)	954928..955542	+	615	WP_029550584.1	hypothetical protein	-
KU531_RS04610 (955981)	955981..956184	+	204	WP_017804629.1	hypothetical protein	-
KU531_RS04615 (956691)	956691..957797	-	1107	WP_000149511.1	tyrosine-type recombinase/integrase	-
KU531_RS04620 (957787)	957787..958590	-	804	WP_000358990.1	helix-turn-helix transcriptional regulator	-
KU531_RS04625 (958726)	958726..958929	+	204	WP_001045296.1	transcriptional regulator	-
KU531_RS04630 (958965)	958965..959183	+	219	WP_000163544.1	helix-turn-helix transcriptional regulator	-
KU531_RS04635 (959195)	959195..959341	+	147	WP_000784885.1	hypothetical protein	-
KU531_RS04640 (959334)	959334..959543	+	210	WP_001058494.1	hypothetical protein	Antitoxin
KU531_RS04645 (959546)	959546..959863	+	318	WP_001103939.1	DUF1474 family protein	Toxin
KU531_RS04650 (959927)	959927..960796	+	870	WP_001002717.1	primase alpha helix C-terminal domain-containing protein	-
KU531_RS04655 (960813)	960813..962270	+	1458	WP_000390453.1	virulence-associated E family protein	-
KU531_RS04660 (962571)	962571..962933	+	363	WP_001039170.1	hypothetical protein	-
KU531_RS04665 (962935)	962935..963219	+	285	WP_000998185.1	hypothetical protein	-
KU531_RS04670 (963216)	963216..963857	+	642	WP_001019808.1	hypothetical protein	-
KU531_RS13650 (964104)	964104..964238	+	135	WP_256094151.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	sec / sell	910168..978027	67859

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1474 (1-98)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12547.17 Da
• Isoelectric Point: 5.0161

>T208645 WP_001103939.1 NZ_CP077925:959546-959863 [Staphylococcus aureus]
MNWEIKDLMCDIEVIKQKINDVATKHAWFVEDRFVKNELETKREHINFSASYLEHRIQNEHTVELLHVYLKEFGELIQKF
HEIEKASSENFGEVSDDAQKLKITE

Nucleotide

• Length: 318 bp

>T208645 NZ_CP103697:c4085536-4085153 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCATCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8268.31 Da
• Isoelectric Point: 4.3718

>AT208645 WP_001058494.1 NZ_CP077925:959334-959543 [Staphylococcus aureus]
MNNEQKEVIEHVVYQLELSVMNNLESYEHTEYVNSIEVVSEISREKHLELIMKWCAQELKNNFQLEKGE

Nucleotide

• Length: 210 bp

>AT208645 NZ_CP103697:c4085778-4085536 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: K7ZRX6

Antitoxin

• AlphaFold DB: X5IY59