Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1123348..1123967 Replicon chromosome
Accession NZ_CP077820
Organism Klebsiella pneumoniae strain FF1013

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KU668_RS05365 Protein ID WP_002892050.1
Coordinates 1123348..1123566 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KU668_RS05370 Protein ID WP_002892066.1
Coordinates 1123593..1123967 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU668_RS05330 (1119395) 1119395..1119658 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
KU668_RS05335 (1119658) 1119658..1119798 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KU668_RS05340 (1119795) 1119795..1120493 - 699 WP_004177238.1 GNAT family protein -
KU668_RS05345 (1120594) 1120594..1122045 + 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
KU668_RS05350 (1122020) 1122020..1122490 - 471 WP_002892026.1 YlaC family protein -
KU668_RS05355 (1122511) 1122511..1122651 + 141 WP_004147370.1 hypothetical protein -
KU668_RS05360 (1122623) 1122623..1123189 - 567 WP_002892030.1 maltose O-acetyltransferase -
KU668_RS05365 (1123348) 1123348..1123566 - 219 WP_002892050.1 HHA domain-containing protein Toxin
KU668_RS05370 (1123593) 1123593..1123967 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KU668_RS05375 (1124453) 1124453..1127599 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KU668_RS05380 (1127622) 1127622..1128815 - 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T207994 WP_002892050.1 NZ_CP077820:c1123566-1123348 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T207994 NZ_CP103557:644211-644525 [Escherichia coli O25b:H4-ST131]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT207994 WP_002892066.1 NZ_CP077820:c1123967-1123593 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT207994 NZ_CP103557:644522-644938 [Escherichia coli O25b:H4-ST131]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References