Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2533417..2534036 Replicon chromosome
Accession NZ_CP077814
Organism Klebsiella pneumoniae strain FF1076

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KU667_RS12250 Protein ID WP_002892050.1
Coordinates 2533417..2533635 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KU667_RS12255 Protein ID WP_002892066.1
Coordinates 2533662..2534036 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU667_RS12215 (KU667_12200) 2529464..2529727 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
KU667_RS12220 (KU667_12205) 2529727..2529867 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KU667_RS12225 (KU667_12210) 2529864..2530562 - 699 WP_002892021.1 GNAT family protein -
KU667_RS12230 (KU667_12215) 2530663..2532114 + 1452 WP_251341118.1 PLP-dependent aminotransferase family protein -
KU667_RS12235 (KU667_12220) 2532089..2532559 - 471 WP_020802585.1 YlaC family protein -
KU667_RS12240 (KU667_12225) 2532580..2532720 + 141 WP_004147370.1 hypothetical protein -
KU667_RS12245 (KU667_12230) 2532692..2533258 - 567 WP_002892030.1 maltose O-acetyltransferase -
KU667_RS12250 (KU667_12235) 2533417..2533635 - 219 WP_002892050.1 HHA domain-containing protein Toxin
KU667_RS12255 (KU667_12240) 2533662..2534036 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KU667_RS12260 (KU667_12245) 2534522..2537668 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KU667_RS12265 (KU667_12250) 2537691..2538884 - 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T207936 WP_002892050.1 NZ_CP077814:c2533635-2533417 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T207936 NZ_CP103544:2703430-2703537 [Escherichia coli]
ATGACGCTCGCGAAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT207936 WP_002892066.1 NZ_CP077814:c2534036-2533662 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT207936 NZ_CP103544:c2703377-2703316 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References